Pituitary component of the aromatic hydrocarbon-mediated expression of CYP2B and CYP2C11.
Pituitary component of the aromatic hydrocarbon-mediated expression of CYP2B and CYP2C11.
复制标题
芳香烃介导的 CYP2B 和 CYP2C11 表达的垂体成分。
DOI：
10.1080/004982598239588
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Backes,WL
中科院分区：
文献类型：
--
作者：
Bergeron,RM;Serron,SC;Rinehart,JJ;Cawley,GF;Backes,WL
1. 目的是确定乙苯 (EB) 介导的 CYP2B 和 CYP2C11 表达是否涉及激素控制成分。 2.用EB治疗垂体切除(HX)和完整大鼠1或2天，并测量对特定CYP水平的影响。 3.与完整对照相比，在HX大鼠中观察到EB对CYP2B的诱导能力存在差异。尽管在完整对照中注射 EB 1 天和 2 天后观察到 CYP2B 依赖性活性和蛋白质水平显着升高，但 HX 大鼠中的 CYP2B 水平仅在碳氢化合物治疗 2 天后才显着升高。 4.对完整大鼠施用EB后，CYP2C11依赖性活性和蛋白质水平均降低。相反，HX 大鼠中的 CYP2C11 水平在指定的任何时间点均不受 EB 的影响。 5.培养的肝细胞中CYP2C11蛋白水平不受EB处理24小时的影响，也支持了激素参与CYP2C11表达的假设。 6. 这项研究表明垂体输入影响 EB 介导的 CYP2B 和 CYP2C11 变化。 CYP2C11 受到 EB 给药的影响，其方式与其他异生素（如苯巴比妥）类似。另一方面，对 EB 的 CYP2B1 2 响应较小的诱导不同于用苯巴比妥观察到的情况，其中 HX 增强了诱导剂的响应。
1. The aim was to determine if the ethylbenzene (EB)-mediated expression of CYP2B and CYP2C11 involved a hormonally controlled component. 2. The hypophysectomized (HX) and intact rats were treated with EB for 1 or 2 days, and the effects on specific CYP levels measured. 3. Differences were observed in the inducibility of CYP2B by EB in the HX rat when compared with intact controls. Whereas significant elevations of CYP2B-dependent activities and protein levels were observed after both 1 and 2 days of EB injection in intact controls, CYP2B levels were significantly elevated in the HX rat only after 2 days of hydrocarbon treatment. 4. Both CYP2C11-dependent activities and protein levels were decreased after EB administration to the intact rat. In contrast, CYP2C11 levels were unaffected by EB in the HX rat at any of the time points indicated. 5. CYP2C11 protein levels were unaffected by treatment with EB for 24 h in cultured hepatocytes, also supporting the hypothesis that hormones are involved in CYP2C11 expression. 6. This studyindicates that pituitary input influences the EB-mediatedchanges inboth CYP2B and CYP2C11. CYP2C11 is affected by EB administration in a manner similar to other xenobiotics such as phenobarbital. On the other hand, the smaller induction of CYP2B1 2 in response to EB differs from that observed with phenobarbital where HX augmented the response of the inducer.