Sepsis and burn complicated by sepsis alter cardiac transporter expression
Sepsis and burn complicated by sepsis alter cardiac transporter expression
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DOI：
10.1016/j.burns.2006.06.009
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发表时间：
2007-02-01
期刊：
影响因子：
2.7
通讯作者：
Horton, Jureta W.
中科院分区：
文献类型：
--
作者：
Ballard-Croft, Cherry;Maass, David L.;Horton, Jureta W.
单纯脓毒症和烧伤合并脓毒症可导致严重的心功能障碍。由于心肌细胞对钙的处理对心脏功能至关重要，因此心脏异常的一种机制可能是钙平衡失调。我们假设脓毒症和烧伤合并脓毒症改变了心脏钙转运蛋白的表达。Sprague-Dawley大鼠分为：(1)对照组，(2)脓毒症（气管内肺炎链球菌，4 × 10(6) CFU），(3)烧伤（40% TBSA）合并脓毒症。分别用Fura-2 AM和SBFI定量心肌细胞[Ca2+](i)和[Na+](i)。大鼠心脏的Western blot分析使用了针对肌浆网状Ca2+ atp酶（SERCA）、l型钙通道、Na+/Ca2+交换器或Na+/K+ atp酶的抗体。结果：脓毒症在存在和不存在烧伤创伤时增加[Ca2+](i)和[Na+](i)。脓毒症组和烧伤合并脓毒症组SERCA表达降低，而钙通道表达在脓毒症组短暂升高。Na+/ Ca2+交换器的表达呈现双相模式。脓毒症和烧伤合并脓毒症降低了Na+/K+ atp酶蛋白水平。这些数据表明，转运蛋白表达的改变会产生心肌细胞钙和钠负荷，并可能导致败血症介导的心脏收缩功能障碍。(C) 2006爱思唯尔有限公司和ISBI。版权所有。
Sepsis alone and burn complicated by sepsis produce significant cardiac dysfunction. Since calcium handling by the cardiomyocyte is essential for cardiac function, one mechanism for cardiac abnormalities may be calcium dyshomeostasis. We hypothesized that sepsis and burn plus sepsis alter cardiac calcium transporter expression. Sprague-Dawley rats were divided into: (1) control, (2) sepsis (intratracheal S. Pneumoniae, 4 x 10(6) CFU), and (3) burn (40% TBSA) plus sepsis. Myocyte [Ca2+](i) and [Na+](i) were quantified with Fura-2 AM and SBFI, respectively. Western blot analysis of rat hearts used antibodies against the sarcoplasmic reticular Ca2+ ATPase (SERCA), the L-type calcium channel, the Na+/Ca2+ exchanger or the Na+/K+ ATPase. RESULTS: Sepsis in the presence and absence of burn trauma increased [Ca2+](i) and [Na+](i). SERCA expression was decreased in the sepsis and burn plus sepsis groups while calcium channel, expression was transiently increased in these sepsis groups. Na+/ Ca2+ exchanger expression exhibited a biphasic pattern of altered expression. Sepsis and burn plus sepsis reduced Na+/K+ ATPase protein levels. These data suggest that altered transporter expression produce cardiomyocyte calcium and sodium loading and may contribute to sepsis-mediated cardiac contractile dysfunction. (C) 2006 Elsevier Ltd and ISBI. All rights reserved.