Effects of exposure to bisphenol A during pregnancy and lactation on the testicular morphology and caspase-3 protein expression of ICR pups
Effects of exposure to bisphenol A during pregnancy and lactation on the testicular morphology and caspase-3 protein expression of ICR pups
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DOI：
10.3892/br.2013.79
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发表时间：
2013-05-01
影响因子：
2.3
通讯作者：
Zhao, Huna
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Li;Chen, Xiao-Yu;Zhao, Huna
双酚A（BPA）是一种异雌激素和内分泌干扰化学品，由于其是一种潜在的人类致癌物而引起关注。本研究旨在探讨BPA对ICR雄性子代睾丸结构和caspase-3蛋白表达的影响及其可能的作用机制。将怀孕的ICR小鼠分为两个对照组和三个治疗组，对照组给予或不给予溶剂二甲基亚砜（DMSO），治疗组从妊娠第0天到出生后第21天（PND）断奶期间灌胃溶于DMSO中的三种不同浓度的水溶性BPA。记录小鼠幼仔数量和雄性与雌性的比例。在PND 21，处死雄性后代以测量其湿重和睾丸系数。电镜观察睾丸形态学变化，Hoechst 33258染色检测睾丸细胞凋亡，免疫组化检测caspase-3表达。对照组和试验组仔鼠在窝产仔数和雌雄比方面差异不显著（P>0.05），但试验组仔鼠睾丸脏器系数降低（P
Bisphenol A (BPA), a xenoestrogen and endocrine-disrupting chemical, is a cause for concern due to its being a potential human carcinogen. The aim of this study was to investigate the effects of continued maternal exposure to BPA on the testicular structures and expression of caspase-3 protein in male ICR offspring during pregnancy and lactation and explore its possible mechanism. Pregnant ICR mice were divided into two control groups, which were either given or not given the solvent dimethyl sulfoxide (DMSO) and three treatment groups, which were gavaged with water-soluble BPA dissolved in DMSO at three different concentrations from gestational day 0 to weaning on postnatal day (PND) 21. The number of mice pups and ratios of males to females were recorded. On PND 21, male offspring were sacrificed to measure their wet weights and testicular coefficients. Electron microscopy was used to observe testicular morphological changes, Hoechst 33258 staining to detect cell apoptosis and immunohistochemistry to measure caspase-3 expression. Although there was no significant difference between offspring of the control group and the treatment group in litter size and male-female ratio (P>0.05), the testicular viscera coefficient in the latter decreased (P