Sensitivity of chronically HIV-1 infected HeLa cells to electrical stimulation
Sensitivity of chronically HIV-1 infected HeLa cells to electrical stimulation
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DOI：
10.1007/s00253-003-1410-8
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发表时间：
2004-02-01
影响因子：
5
通讯作者：
Harada, S
中科院分区：
文献类型：
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作者：
Kumagai, E;Tominaga, M;Harada, S
使用包括蛋白酶抑制剂在内的联合抗逆转录病毒药物治疗方案可显着降低 HIV-1 感染者的发病率和死亡率。然而，除了出现耐药性 HIV-1 毒株之外，这种联合疗法似乎还有许多副作用。因此，在这项研究中，我们试图阐明针对 HIV-1 感染的新治疗原则。我们研究了电刺激对长期感染 HIV-1(LAI) 的 HeLa 细胞 (P6 HeLa/HIV-1(LAI)) 和未感染细胞 (P6 HeLa) 的影响。将细胞培养在光学透明电极上，并在 10 分钟至 60 分钟的时间内施加相对于 Ag/AgCl 1.0 V 的电位。随着电刺激持续时间的增加，P6 HeLa/HIV-1（LAI）和 P6 HeLa 细胞逐渐受损。然而，P6 HeLa/HIV-1(LA1)细胞比P6 HeLa细胞更容易受到电刺激的影响。电刺激30分钟时损伤率差异最为明显，损伤细胞分别占P6 HeLa/HIV-1(LAI)和P6 HeLa细胞的87%和4%。施加电位20 min后，P6 HeLa/HIV-1（LAI）细胞的增殖受到明显抑制，而P6 HeLa细胞的增殖程度与未施加电位的未感染细胞相似。这些结果表明，长期感染 HIV-1 的细胞对电刺激的敏感性比未感染的细胞高得多。这可以被认为是对抗 HIV-1 感染的一种有用的新方法。
Use of combination anti-retroviral drug regimens including protease inhibitors dramatically decreased morbidity and mortality rates in HIV-1 infected individuals. However, such combination therapies appear to have many side effects, in addition to the emergence of resistant HIV-1 strains. Therefore, in this study we sought to elucidate novel therapeutic principles against HIV-1 infection. We examined the effects of electrical stimulation on both chronically HIV-1(LAI) infected HeLa cells (P6 HeLa/HIV-1(LAI)) and uninfected cells (P6 HeLa). Cells were cultured on an optically transparent electrode and application of potential at 1.0 V vs Ag/AgCl was performed over time periods ranging from 10 min to 60 min. Both P6 HeLa/HIV-1(LAI) and P6 HeLa cells were progressively damaged as the duration of electrical stimulation increased. However, P6 HeLa/HIV-1(LAl) cells were much more influenced by electrical stimulation than P6 HeLa cells. The difference in damage rate was most obvious at 30 min of electrical stimulation, with damaged cells accounting for about 87% and 4% of P6 HeLa/HIV-1(LAI) and P6 HeLa cells, respectively. After the application of potential for 20 min, the proliferation of P6 HeLa/HIV-1(LAI) cells was markedly inhibited, while the P6 HeLa cells proliferated to an extent similar to that of uninfected cells without application of potential. These results indicate that sensitivity to electrical stimulation is much higher in chronically HIV-1 infected cells than in uninfected cells. This could be considered as a useful new approach against HIV-1 infection.