Genetic dissection of plant cell-wall biosynthesis
Genetic dissection of plant cell-wall biosynthesis
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DOI：
10.1042/bst0300298
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发表时间：
2002-04-01
影响因子：
3.9
通讯作者：
Somerville, CR
中科院分区：
文献类型：
--
作者：
Bonetta, DT;Facette, M;Somerville, CR
植物细胞壁是由多种聚合物组成的复杂结构，包括纤维素、木葡聚糖、木聚糖和聚半乳糖醛酸聚糖。生物化学和遗传分析使得克隆编码纤维素酶（CesA）的基因成为可能。在拟南芥基因组中预测的蛋白质序列的比较表明，存在30个不同的基因与相似的CesAs。这些纤维素酶样（Csl）蛋白可能不有助于纤维素合成，而是有助于其他壁聚合物的合成。因此，一个主要的挑战是为这些基因分配生物学功能。为了解决这个问题，我们系统地鉴定了17个拟南芥Csls中的T-DNA或转座子插入。“敲除”突变体的表型表征包括通过傅立叶变换红外显微镜测定突变体细胞壁与野生型植物的光谱特征差异。更精确的表征包括细胞壁分级分离，然后通过阴离子交换色谱法进行中性糖组成分析。
The plant cell wall is a complex structure consisting of a variety of polymers including cellulose, xyloglucan, xylan and polygalacturonan. Biochemical and genetic analysis has made it possible to clone genes encoding cellulose synthases (CesA). A comparison of the predicted protein sequences in the Arabidopsis genome indicates that 30 divergent genes with similarity to CesAs exist. It is possible that these cellulose synthase-like (Csl) proteins do not contribute to cellulose synthesis, but rather to the synthesis of other wall polymers. A major challenge is, therefore, to assign biological function to these genes. In an effort to address this issue we have systematically identified T-DNA or transposon insertions in 17 Arabidopsis Csls. Phenotypic characterization of 'knock-out' mutants includes the determination of spectroscopic profile differences in mutant cell walls from wild-type plants by Fourier-transform IR microscopy. A more precise characterization includes cell wall fractionation followed by neutral sugar composition analysis by anionic exchange chromatography.