Uroplakin III-Delta4 messenger RNA as a promising marker to identify nonulcerative interstitial cystitis
Uroplakin III-Delta4 messenger RNA as a promising marker to identify nonulcerative interstitial cystitis
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DOI：
10.1016/j.juro.2007.05.125
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发表时间：
2007-10-01
影响因子：
6.6
通讯作者：
Kakehi, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Zeng, Yu;Wu, Xiu-Xian;Kakehi, Yoshiyuki
目的：间质性膀胱炎仍然是一个了解不多的泌尿系统疾病，其特征是慢性盆腔疼痛和尿频，缺乏任何已知的病因。尿路上皮功能障碍和其他异常被认为是参与疾病。由尿路上皮细胞表达的尿斑蛋白被认为是尿路上皮顶端表面的主要屏障蛋白，具有重要作用。材料和方法：采用实时逆转录-聚合酶链反应定量测定了29例间质性膀胱炎患者和16例对照组膀胱活检标本中尿斑蛋白Ia、Ib、II、III和III-delta 4的基因表达。与正常对照组相比，间质性膀胱炎膀胱中尿斑蛋白Ia、Ib和II基因的mRNA水平相对较低，尿斑蛋白III相对较高，但不显著。尿斑蛋白III-δ 4是尿斑蛋白III的剪接变体，在间质性膀胱炎样本中显著上调（p
Purpose: Interstitial cystitis remains a poorly understood urological condition characterized by chronic pelvic pain and increased urinary frequency in the absence of any known etiology. Urothelial dysfunction and other abnormalities are presumed to be involved in the disease. Uroplakins that are expressed by urothelial cells are thought to have an important role as major barrier proteins on the apical surface of the urothelium.Materials and Methods: Gene expression of uroplakin Ia, Ib, II, III and III-delta4 was quantitatively measured in bladder biopsy samples from 29 patients with interstitial cystitis and 16 control subjects using real-time reverse transcriptase-polymerase chain reaction.Results: The mRNA levels of the uroplakin Ia, Ib and II genes were relatively low and uroplakin III was relatively high in interstitial cystitis bladders compared to normal controls, although not significantly. Uroplakin III-delta4, a splicing variant of uroplakin III, was significantly up-regulated in interstitial cystitis samples (p