MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions
MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions
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DOI：
10.1007/bf00702348
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发表时间：
1996-10-01
影响因子：
3
通讯作者：
Carlstedt, I
中科院分区：
文献类型：
--
作者：
Hovenberg, HW;Davies, JR;Carlstedt, I
从健康和慢性支气管炎受试者收集气道粘液。慢性支气管炎痰液经离心分离成凝胶相和溶胶相，用氯化铯等密度梯度离心分离粘蛋白。MUC5AC和MUC2粘蛋白的存在用抗血清研究，所述抗血清针对具有来自相应脱辅基蛋白的序列的合成肽产生。慢性支气管炎痰液的凝胶和溶胶相以及健康的呼吸道分泌物显示含有MUC5AC，而MUC2粘蛋白不能被检测到。速率区带离心表明，MUC5AC粘蛋白是大的，多分散的大小和减少产生亚基。离子交换HPLC显示，在所有的分泌物中存在两个亚基群体，MUC5AC亚基总是更酸性。因此，MUC5AC是第一个鉴定的大的、基于亚基的、形成凝胶的呼吸道粘蛋白，并且这种糖蛋白在生物化学上不同于至少一种也由亚基组成但缺乏遗传同一性的其它大的、形成凝胶的粘蛋白群体。
Airway mucus was collected from healthy and chronic bronchitic subjects. The chronic bronchitic sputum was separated into gel and sol phase by centrifugation and mucins were isolated using isopycnic density-gradient centrifugation in CsCl. The presence of the MUC5AC and MUC2 mucins was investigated with antisera raised against synthetic peptides with sequences from the respective apoproteins. The gel and sol phase of chronic bronchitic sputum as well as healthy respiratory secretions were shown to contain MUC5AC whereas the MUC2 mucin could not be detected. Rate-zonal centrifugation showed that the MUC5AC mucin was large, polydisperse in size and that reduction yielded subunits. Ion-exchange HPLC revealed the presence of two subunit populations in all secretions, the MUC5AC subunits always being the more acidic. MUC5AC is thus the first large, subunit-based, gel-forming respiratory mucin identified and this glycoprotein is biochemically distinct from at least one other population of large, gel-forming mucins also composed of subunits but lacking a genetic identity.