Binding and neutralization characteristics of a panel of monoclonal antibodies to human papillomavirus 58
Binding and neutralization characteristics of a panel of monoclonal antibodies to human papillomavirus 58
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DOI：
10.1099/vir.0.017228-0
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发表时间：
2010-07-01
影响因子：
3.8
通讯作者：
Christensen, Neil D.
中科院分区：
文献类型：
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作者：
Brendle, Sarah A.;Culp, Timothy D.;Christensen, Neil D.
人乳头瘤病毒(HPV)58是一种与侵袭性生殖器癌进展相关的高危HPV类型。我们研制了六种抗HPV58 L1病毒样颗粒的单抗，可结合HPV58上的构象表位。杂交瘤细胞系适应于无血清和无动物成分的条件，并对mAb上清液进行亲和纯化。这6株单抗对HPV58假病毒(PSV)和准病毒粒子具有不同的中和能力。这些单抗在阻止PsV58与HaCaT细胞、HaCaT细胞沉积的细胞外基质(ECM)、肝素和纯化的人层粘连蛋白5(ECM)之间的黏附能力上存在差异。这些单抗提供了一套独特的工具来研究以前未经测试的高危HPV类型的结合特性，并有机会将这些特性与其他HPV类型的结合进行比较。
Human papillomavirus (HPV) 58 is a high-risk HPV type associated with progression to invasive genital carcinomas. We developed six monoclonal antibodies (mAbs) against HPV58 L1 virus-like particles that bind conformational epitopes on HPV58. The hybridoma cell lines were adapted to serum- and animal component-free conditions and the mAb supernatants were affinity-purified. The six mAbs neutralized HPV58 pseudoviruses (PsVs) and 'quasivirions' with different capacities. The mAbs differed in their ability to prevent PsV58 attachment to HaCaT cells, to the extracellular matrix (ECM) deposited by HaCaT cells, to heparin and to purified human laminin 5, a protein in the ECM. These mAbs provide a unique set of tools to study the binding properties of a previously untested, high-risk HPV type and the opportunity to compare these characteristics with the binding of other HPV types.