CYTOGENETICS OF UNDIFFERENTIATED NASOPHARYNGEAL CARCINOMA XENOGRAFTS FROM SOUTHERN CHINESE
CYTOGENETICS OF UNDIFFERENTIATED NASOPHARYNGEAL CARCINOMA XENOGRAFTS FROM SOUTHERN CHINESE
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DOI：
10.1002/ijc.2910430535
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发表时间：
1989-05-15
影响因子：
6.4
通讯作者：
LUI, M
中科院分区：
文献类型：
--
作者：
HUANG, DP;HO, JHC;LUI, M
用新鲜活检组织经皮下注射成功地建立了三株未分化鼻咽癌（NPC）肿瘤细胞系。植入无胸腺小鼠体内并传代许多代。发现这些异种移植物不含浸润性淋巴样细胞，并且直至第30代仍保持未分化。它们分别被设计为NPC/HK 2117（Xeno-I）、NPC/HK 1915（Xeno-2）和NPC/HK 1530（Xeno-3）。采用胰蛋白酶-Giemsa法G显带，对Xeno-I的第16代、Xeno-2的第I代和Xeno-3的第14代进行细胞遗传学研究。两个异种移植物为超二倍体，染色体众数范围为49至76，一个为亚二倍体，众数范围为28至38。已鉴定出5条标记染色体，涉及染色体1、3、11、12和17。其中1例移植瘤的标记染色体为12 q、1 q和3q，另1例移植瘤的标记染色体为12、11和17。6个标记中的3个涉及12 q13 →。qter。还观察到一条异常的3号染色体，其中大部分p臂缺失。
Three undifferentiated nasopharyngeal carcinoma (NPC) tumour lines were successfully established from fresh biopsy material injected s.c. into athymic mice and passaged for many generations. These xenografts were found to be free of infiltrating lymphoid cells and remained undifferentiated up to passage 30. They were designed NPC/HK2117 (Xeno-I), NPC/HK1915 (Xeno-2) and NPC/HK1530 (Xeno-3), respectively. Passage 16 from Xeno-I, passage I from Xeno-2 and passage 14 from Xeno-3 were studied cytogenetically using G-banding with the trypsin-Giemsa method. Two xenografts were hyperdiploid with chromosome modal numbers ranging from 49 to 76, and one was hypodiploid with modal numbers ranging from 28 to 38. Five marker chromosomes have been identified with involvement of chromosomes 1, 3, 11, 12 and 17. Marker chromosomes derived from chromosomes 12q, 1q and 3q were consistent in one of the 2 xenografts successfully G-banded, and chromosomes 12, 11 and 17 were consistent in another. Three out of the 6 markers involve 12q13 .fwdarw. qter. An abnormal chromosome 3 with most of the p arm deleted was also observed.