Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development.
Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development.
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DOI：
10.1002/anie.201500342
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发表时间：
2015-05-11
影响因子：
16.6
通讯作者：
Tate, Edward W.
中科院分区：
文献类型：
--
作者：
Broncel, Malgorzata;Serwa, Remigiusz A.;Ciepla, Paulina;Krause, Eberhard;Dallman, Margaret J.;Magee, Anthony I.;Tate, Edward W.
新型多功能试剂与脂质探针结合应用于豆蔻酰化蛋白的亲和富集和通过质谱法直接检测脂质修饰的胰蛋白酶肽。该方法能够在细胞培养中以前所未有的规模高置信度鉴定肉豆蔻酰化蛋白质组，并首次定量分析脊椎动物胚胎发育过程中蛋白质脂质化的动态变化。
Novel multifunctional reagents were applied in combination with a lipid probe for affinity enrichment of myristoylated proteins and direct detection of lipid-modified tryptic peptides by mass spectrometry. This method enables high-confidence identification of the myristoylated proteome on an unprecedented scale in cell culture, and allowed the first quantitative analysis of dynamic changes in protein lipidation during vertebrate embryonic development.
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影响因子：
16.6
作者：
Thinon, Emmanuelle;Serwa, Remigiusz A.;Broncel, Malgorzata;Brannigan, James A.;Brassat, Ute;Wright, Megan H.;Heal, William P.;Wilkinson, Anthony J.;Mann, David J.;Tate, Edward W.
通讯作者：
Tate, Edward W.
DOI：
10.1007/s12154-009-0032-8
发表时间：
2010-03-01
期刊：
Journal of chemical biology
影响因子：
--
作者：
Wright, Megan H;Heal, William P;Tate, Edward W
通讯作者：
Tate, Edward W
影响因子：
15
作者：
Speers, AE;Cravatt, BF
通讯作者：
Cravatt, BF
影响因子：
21.8
作者：
Wright MH;Clough B;Rackham MD;Rangachari K;Brannigan JA;Grainger M;Moss DK;Bottrill AR;Heal WP;Broncel M;Serwa RA;Brady D;Mann DJ;Leatherbarrow RJ;Tewari R;Wilkinson AJ;Holder AA;Tate EW
通讯作者：
Tate EW
影响因子：
14.8
作者：
Heal, William P.;Wright, Megan H.;Tate, Edward W.
通讯作者：
Tate, Edward W.