REGIONAL AND LAMINAR DISTRIBUTION OF THE DOPAMINE AND SEROTONIN INNERVATION IN THE MACAQUE CEREBRAL-CORTEX - A AUTORADIOGRAPHIC STUDY
REGIONAL AND LAMINAR DISTRIBUTION OF THE DOPAMINE AND SEROTONIN INNERVATION IN THE MACAQUE CEREBRAL-CORTEX - A AUTORADIOGRAPHIC STUDY
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DOI：
10.1002/cne.902730109
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发表时间：
1988-07-01
影响因子：
2.5
通讯作者：
ALVAREZ, C
中科院分区：
文献类型：
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作者：
BERGER, B;TROTTIER, S;ALVAREZ, C
利用放射自显影技术研究了食蟹猴大脑皮层多巴胺（DA）和5-羟色胺（5-HT）传入神经元的区域密度和层状分布，该技术基于这些胺能神经元的高亲和力摄取能力。将50 μ m厚的大振动切片机切片与[3 H] DA（0.2 μ M）和地昔帕明（5 μ M）或与未标记的去甲肾上腺素（5 μ M）和[3 H] 5-HT（0.6 μ M）一起温育，这允许分别特异性标记DA和5-HT神经支配。固定后，将这些切片干燥、脱脂，并通过浸渍进行放射性签名。通过对epon包埋后获得的切片的4 μ m厚切片的放射自显影照片中的[3 H] DA标记的轴突静脉曲张进行计数，也提供了关于DA神经支配的半定量数据。DNA神经分布广泛，在无颗粒皮质和颗粒皮质的密度和层状分布不同。DA传入在前扣带回（24区）和运动区（4区、6区和辅助运动区[SMA]）中是密集的。在后者中，它们显示出三层的分布模式，主要分布在I、IIIa和V-VI层，在IIIa层中具有特征性的簇状结构，特别是在运动区的内侧部分。在颗粒状前额叶（区域46、9、10、11、12），顶骨（区域1、2、3、5、7），颞叶（21，22区）和后扣带回（23区），DA传入纤维密度较低，呈双层分布，主要分布在I层和V-VI层; Ⅱ、Ⅲ、Ⅳ层的密度仅为Ⅱ、Ⅲ、Ⅳ层的20%。I.密度最低的是视皮层，特别是在17区，DA传入几乎仅限于第一层。除运动区和前扣带回外，5-HT神经支配的密度普遍大于DA神经支配的密度。区域特异性层状模式的特征在于（1）运动区，其中层III中的较低密度与同一层中的DA轴突簇形成对比;（2）初级视觉皮层（区域17），其中层III-IV和层V中的两个较高密度的带勾勒出层IVc-β中神经支配不良的区域; (3)18周纹区，5-HT网络相对松散，但在第III层中具有较密集的带。因此，DA神经支配的大脑皮层显示啮齿类动物和灵长类动物之间的主要差异，其特征在于扩大皮质的目标和高度分化的层状分布。这表明在运动和更高级的大脑功能的控制中，皮层水平的DNA具有更大的功能重要性和直接作用。
The regional density and laminar distribution of dopamine (DA) and serotonin (5-HT) afferents were investigated in the cerebral cortex of cynomolgus monkeys using a radioautographic technique that is based on the high affinity uptake capacity of these aminergic neurons. Large vibratome sections, 50 .mu.m thick, were incubated with [3H] DA (0.2 .mu.M) and desipramine (5 .mu.M) or with unlabeled norepinephrine (5 .mu.M) and [3H] 5-HT (0.6 .mu.M), which allowed for the specific labeling of the DA and 5-HT innervations, respectively. After fixation, these sections were dried, defatted, and radioautographed by dipping. Semiquantitative data on the DA innervation also were provided by counting [3H] DA-labeled axonal varicosities in radioautographs from 4-.mu.m-thick sections of the slices obtained after epon embedding. The DNA innervation was widespread and differed in density and laminar distribution in the agranular and granular cortices. DA afferents were densest in the anterior cingulate (area 24) and the motor areas (areas 4, 6, and supplementary motor area [SMA]). In the latter they displayed a trilaminar pattern of distriubtion, predominating in layers I, IIIa, and V-VI, with characteristic cluster-like formations in layer IIIa, especially in the medial part of motor areas. In the granular prefrontal (areas 46, 9, 10, 11, 12), parietal (areas 1, 2, 3, 5, 7), temporal (areas 21, 22), and posterior cingulate (area 23) cortices, DA afferents were less dense and showed a bilaminar pattern of distribution, predominating in the depth of layer I and in layers V-VI; density in layers II, III, and IV was only 20% of that in layer. I. The lowest density was in the visual cortex, particularly in area 17, where the DA afferents were almost restricted to layer I. The density of 5-HT innervation was generally greater than that of DA except in the motor areas and in the anterior cingulate cortex. Region specific laminar patterns characterized (1) motor areas where a lower density in layer III contrasted with the clusters of DA axons in the same layer; (2) the primary visual cortex (area 17), where two bands of higher density in layers III-IV and layer V outlined a poorly innervated zone in layer IVc-.beta.; (3) the peristriate area 18, where the 5-HT network was relatively loose but with a denser band in layer III. Thus, DA innervation of the cerebral cortex displays major differences between rodents and primates, characterized by expanded cortical targets and by a highly differentiated laminar distribution. This suggests a greater functional importance and a direct role of DNA at the cortical level in the control of motor and higher order brain functions.