Responsive membranes for hydrophobic interaction chromatography
Responsive membranes for hydrophobic interaction chromatography
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DOI：
10.1016/j.memsci.2013.07.020
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发表时间：
2013-11-15
影响因子：
9.5
通讯作者：
Wickramasinghe, S. Ranil
中科院分区：
文献类型：
--
作者：
Himstedt, Heath H.;Qian, Xianghong;Wickramasinghe, S. Ranil
采用原子转移自由基聚合（ATRP）技术在再生纤维素膜表面生长聚n -乙烯基己内酰胺（PVCL）链。利用扫描电镜、红外光谱对改性膜进行了表征。x射线光电子能谱和表面接触角的测量。随着ATRP引发剂固定时间的延长，PVCL接枝度增加。PVCL是一种热敏聚合物，具有较低的临界溶液温度（LCST），该温度取决于溶液中盐离子的浓度。在含有1.8 M (NH3)(2)SO4的缓冲液中，LCST在室温下降低，聚合物采用更疏水/坍塌的构象。在低离子强度下，LCST保持在室温以上。测定了PVCL在含1.8 M (NH3)(2)SO4和不含1.8 M (NH3)(2)SO4的20 mM磷酸盐缓冲液中的接触角。高离子强度缓冲液的接触角大于低离子强度缓冲液。研究了牛血清白蛋白（BSA）和免疫球蛋白G （lgG）的吸附和解吸。负载在高离子强度缓冲液中进行。在低离子强度缓冲液中进行洗脱。通过使用在加载和洗脱过程中改变其构象的反应性配体，获得了高的蛋白质回收率。响应溶液离子强度的配体有望用于高性能疏水相互作用色谱。(c) 2013 Elsevier B.V.版权所有
Poly N-vinylcaprolactam (PVCL) chains were grown from the surface of regenerated cellulose membranes using atom transfer radical polymerization (ATRP). Modified membranes were characterized using scanning electron microscopy, infrared spectroscopy. X-ray photoelectron spectroscopy and by measuring surface contact angles. The grafting degree of PVCL increases with increasing ATRP initiator immobilization time. PVCL is a thermo-responsive polymer with a lower critical solution temperature (LCST) that depends On the concentration of salt ions present in solution. The LCST decreases below room temperature in buffer containing 1.8 M (NH3)(2)SO4 where the polymer adopts a more hydrophobic/collapsed conformation. At low ionic strength, the LCST remains above room temperature. Contact angles for PVCL in 20 mM phosphate buffer with and without 1.8 M (NH3)(2)SO4 were determined. Contact angles in the buffer with high ionic strength were higher than those in low ionic strength.Adsorption and desorption of bovine serum albumin (BSA) and immunoglobulin G (lgG) have been investigated. Loading was conducted in high ionic strength buffer. Elution was conducted in low ionic strength buffer. By using a responsive ligand that changes its conformation during loading and elution, high protein recoveries were obtained. Ligands that respond to solution ionic strength show promise for high performance hydrophobic interaction chromatography. (c) 2013 Elsevier B.V. All rights reserved