Monodisperse boronate polymeric particles synthesized by a precipitation polymerization strategy: particle formation and glycoprotein response from the standpoint of the Flory-Huggins model.
Monodisperse boronate polymeric particles synthesized by a precipitation polymerization strategy: particle formation and glycoprotein response from the standpoint of the Flory-Huggins model.
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DOI：
10.1021/am405144x
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发表时间：
2014-01
影响因子：
9.5
通讯作者：
Jianxi Liu;Yanyan Qu;Kaiguang Yang;Qi Wu;Y. Shan;Lihua Zhang;Zhenhua Liang;Yukui Zhang
中科院分区：
文献类型：
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作者：
Jianxi Liu;Yanyan Qu;Kaiguang Yang;Qi Wu;Y. Shan;Lihua Zhang;Zhenhua Liang;Yukui Zhang
从复杂生物样品中捕获糖蛋白的高特异性识别系统的开发是基于质谱学的糖蛋白组学分析成功的先决条件。为实现这一目的，以4-乙烯基苯基硼酸为功能单体，N，N‘-亚甲基双丙烯酰胺为交联剂(S)为交联剂，采用水/乙醇(4：1，v/v)组成的新型溶剂体系，采用一锅沉淀法(PP)制备了硼酸盐亲和聚合物微米/纳米粒子。所提出的聚合策略不仅提供了表面光滑、尺寸可控的单分散亚微米级聚合物粒子，而且增加了硼酸的负载量，这是其他聚合方法难以实现的，从而导致对糖蛋白的特异性识别。在Flory-Huggins模型的基础上，进一步考察了溶剂体系和单体对合成材料的形貌和亲硼能力的影响。实验证明，各种单体在颗粒形成过程中的反应速度可能是影响糖蛋白亲和力的关键因素。我们的结果表明，在Flory-Huggins模型的理论指导下，选择合适的单体和溶剂体系的PP策略可能为制备表面含有丰富的硼酸基团的亚微米级聚合物粒子提供了一种很好的途径，从而实现了对糖蛋白的高选择性浓缩。
The development of a highly specific recognition system for glycoprotein capture from complex biological samples is a prerequisite to the success of mass spectra-based glycoproteomics analysis. To achieve this purpose, a one-pot precipitation polymerization (PP) strategy with a novel solvent system composed of water/ethanol (4:1, v/v) is described for preparing boronate-affinity polymeric micro/nano particles using 4-vinylphenylboronic acid (VPBA) as the functional monomer and N,N'-methylenebis(acrylamide) (MBA) alone or together with divinylbenzene (DVB) as the cross-linker(s). The proposed polymerization strategy not only affords monodisperse polymeric submicrometer particles with a smooth surface and controllable size, ranging from 300 to 700 nm, but also increases the loading capacity of boronic acid, which could hardly be achieved by other polymerization methods, thus leading to the specific recognition of glycoproteins. The effects of solvent systems and monomers on the morphology and boronate-affinity capacity of prepared materials were further investigated based on the Flory-Huggins model. It was proved that the reaction rate of various monomers during particle formation might be the key factor affecting the affinity capacity for glycoproteins. Our results demonstrated that under the theoretical guidance of the Flory-Huggins model the PP strategy with a selected monomer and solvent system might provide a good approach to prepare submicrometer polymer particles with plenty of boronic acid groups on the surface to achieve a highly selective enrichment of glycoproteins.