EARLY EVENTS IN THE INFECTION OF SOYBEAN (GLYCINE-MAX L MERR) BY RHIZOBIUM-JAPONICUM .1. LOCALIZATION OF INFECTIBLE ROOT-CELLS
EARLY EVENTS IN THE INFECTION OF SOYBEAN (GLYCINE-MAX L MERR) BY RHIZOBIUM-JAPONICUM .1. LOCALIZATION OF INFECTIBLE ROOT-CELLS
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DOI：
10.1104/pp.66.6.1027
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发表时间：
1980-01-01
期刊：
影响因子：
7.4
通讯作者：
BAUER, WD
中科院分区：
文献类型：
--
作者：
BHUVANESWARI, TV;TURGEON, BG;BAUER, WD
大豆根系的侵染细胞似乎在任何给定时间都位于根伸长区正上方和最小出芽根毛的位置正下方。接种时感染细胞在初生根上的位置是根据随后的根瘤发育位置推断出来的，这纠正了由于根伸长而导致的表皮细胞位移。接种时在幼苗生长袋上做标记，表明根尖位置和根毛发育区域。几乎所有的幼苗在接种时根尖上的标记上方的主根上都长出了根瘤。接种时，在有成熟根毛的地方，没有一株形成根瘤。这些结果和结节频率的分布表明，感染细胞的位置是发育受限的。标记根尖位置后延迟接种1 ~ 4小时，根尖标记上方形成的根瘤数量逐渐减少，根尖标记上方形成的根瘤数量逐渐减少。给定宿主细胞的传染性可能是一种短暂的特性，在几个小时内出现并消失。大多数导致感染和结瘤的反应可在接种后不到2小时内触发或开始。根尖标记以上结瘤的程度与接种物中细菌数量的对数成比例地增加。
The infectible cells of soybean roots appear to be located at any given time just above the zone of root elongation and just below the position of the smallest emergent root hairs. The location of infectible cells on the primary root at the time of inoculation was inferred from the position of subsequent nodule development, correcting for displacement of epidermal cells due to root elongation. Marks were made on the seedling growth pouches at the time of inoculation to indicate the position of the root tip and the zones of root hair development. Virtually all of the seedlings developed nodules on the primary root above the marks made at the root tips at the time of inoculation. None of the plants formed nodules on the root where mature root hairs were present at the time of inoculation. These results and profiles of nodulation frequency indicate that the location of infectible cells is developmentally restricted. When inoculations were delayed for intervals of 1-4 h after marking the positions of the root tips, progressively fewer nodules were formed above the root tip marks, and the uppermost of these nodules were formed at progressively shorter distances above the marks. The infectibility of given host cells may be a transient property that appears and is lost within a few hours. Most responses leading to infection and nodulation may be triggered or initiated in less than 2 h after inoculation. The extent of nodulation above the root tip mark increased in proportion to the logarithm of the number of bacteria in the inoculum.