Duration of life-cycle toxicity tests with the ostracod Heterocypris incongruens
Duration of life-cycle toxicity tests with the ostracod Heterocypris incongruens
复制标题
介形类异鲤生命周期毒性试验的持续时间
DOI：
10.1002/etc.3931
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发表时间：
2017
影响因子：
4.1
通讯作者：
Tsukahara Kenta
中科院分区：
文献类型：
--
作者：
Hiki Kyoshiro;Tobino Tomohiro;Nakajima Fumiyuki;Tsukahara Kenta
使用介形虫Heterocypris incongruensis进行的急性沉积物毒性试验用户友好且灵敏度高;然而，尚未开发使用该物种的生命周期试验。开发生命周期测试时最具挑战性的问题是卵子发育时间差异很大，有时太长（> 150天）无法监测。希望缩短生命周期毒性试验的持续时间，包括卵发育的观察期，同时保留净繁殖率（R 0）的生态相关性，这是生命周期试验的终点。因此，在本研究中，我们建议一个实用的测试时间为R 0使用人口增长率（λ）作为生态相关性的措施。结合文献资料和实验结果，收集了介形虫的生活史特征，构建了介形虫种群矩阵模型，计算了20种生活史模式的λ。结果表明，延长试验周期（> 150 d）并不增加R 0与λ的相关系数。相反，较短的测试持续时间导致R 0与λ高度相关。我们的研究结果表明，使用介形虫的生命周期毒性测试可以提供生态相关的毒性终点，即使测试在大约50天后被放弃并且未孵化的卵仍然存在。Environ Toxicol Chem 2017;36：3443-3449。联系我们_SETAC
An acute sediment toxicity test using the ostracodHeterocypris incongruensis user friendly and has high sensitivity; however, a life‐cycle test using this species has not been developed. The most challenging problem when developing a life‐cycle test is that the egg development time varies greatly and is sometimes too long (> 150 d) to monitor. It is desirable to shorten the duration of life‐cycle toxicity tests including the observation period of egg development while preserving the ecological relevance of the net reproductive rate (R0), an endpoint in the life‐cycle test. Therefore, in the present study, we suggest a practical test duration forR0using population growth rate (λ) as a measure of ecological relevance. We collected a range of life history characteristics of the ostracod by combining data from the literature and experimental results, constructed population matrix models, and calculatedλfor 20 life history patterns. The results showed that a longer test period (> 150 d) did not increase the correlation coefficients betweenR0andλ. Rather, a shorter test duration resulted inR0being highly correlated withλ. Our results suggest that a life‐cycle toxicity test using the ostracod can provide an ecologically relevant toxicity endpoint, even if the test is abandoned after approximately 50 d and unhatched eggs remain.Environ Toxicol Chem2017;36:3443–3449. © 2017 SETAC