Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells.
Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells.
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DOI：
10.1038/srep35474
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发表时间：
2016-10-14
影响因子：
4.6
通讯作者：
Di Carlo D
中科院分区：
文献类型：
--
作者：
Dhar M;Pao E;Renier C;Go DE;Che J;Montoya R;Conrad R;Matsumoto M;Heirich K;Triboulet M;Rao J;Jeffrey SS;Garon EB;Goldman J;Rao NP;Kulkarni R;Sollier-Christen E;Di Carlo D
循环肿瘤细胞（CTC）作为转移性疾病的指标具有很大的潜力，可以帮助医生改善癌症的诊断、治疗和患者的预后。异质性标志物表达以及目前基于抗体的分离和分析系统的复杂性突出了对替代方法的需求。在这项工作中，我们使用了一种微流体涡流装置，可以选择性地从血液中分离出潜在的肿瘤细胞，而不依赖于细胞表面的表达。该系统适用于与三种无蛋白质标记物分析技术接口：（i）流式自动图像处理系统，用于计数释放的细胞，（ii）使用巴氏（Pap）染色的细胞学分析和（iii）靶向ALK重排的荧光原位杂交（FISH）。入流计数能够在几分钟内快速评估样本中的癌症相关大循环细胞，以确定是否需要标准的下游检测，例如更耗时和昂贵的细胞学和细胞遗传学分析。使用我们的平台与这些工作流程集成，我们分析了32个非小细胞肺癌（NSCLC）和22个乳腺癌患者样本，根据所使用的检测方法，60 - 100%的癌症患者的细胞计数超过健康阈值：分别为77.8%的自动化，60-100%的细胞学和80%的基于免疫染色的计数。
Circulating tumor cells (CTCs) have a great potential as indicators of metastatic disease that may help physicians improve cancer prognostication, treatment and patient outcomes. Heterogeneous marker expression as well as the complexity of current antibody-based isolation and analysis systems highlights the need for alternative methods. In this work, we use a microfluidic Vortex device that can selectively isolate potential tumor cells from blood independent of cell surface expression. This system was adapted to interface with three protein-marker-free analysis techniques: (i) an in-flow automated image processing system to enumerate cells released, (ii) cytological analysis using Papanicolaou (Pap) staining and (iii) fluorescence in situ hybridization (FISH) targeting the ALK rearrangement. In-flow counting enables a rapid assessment of the cancer-associated large circulating cells in a sample within minutes to determine whether standard downstream assays such as cytological and cytogenetic analyses that are more time consuming and costly are warranted. Using our platform integrated with these workflows, we analyzed 32 non-small cell lung cancer (NSCLC) and 22 breast cancer patient samples, yielding 60 to 100% of the cancer patients with a cell count over the healthy threshold, depending on the detection method used: respectively 77.8% for automated, 60–100% for cytology, and 80% for immunostaining based enumeration.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
14.8
作者：
通讯作者：
--
DOI：
10.1126/science.1235226
发表时间：
2013-09-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Plaks V;Koopman CD;Werb Z
通讯作者：
Werb Z
影响因子：
45.3
作者：
Pailler, Emma;Adam, Julien;Farace, Francoise
通讯作者：
Farace, Francoise
影响因子：
11.5
作者：
Riethdorf, Sabine;Fritsche, Herbert;Pantel, Klaus
通讯作者：
Pantel, Klaus