Avian hemangioma retrovirus induces cell proliferation via the envelope (env) gene
Avian hemangioma retrovirus induces cell proliferation via the envelope (env) gene
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DOI：
10.1006/viro.2000.0550
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发表时间：
2000-10-10
期刊：
影响因子：
3.7
通讯作者：
Eldor, A
中科院分区：
文献类型：
--
作者：
Alian, A;Sela-Donenfeld, D;Eldor, A
几年前，从蛋鸡血管瘤瘤中分离到一株反转录病毒，即禽血管瘤病毒（avian hemangioma Virus，AHV）。序列分析表明，AHV基因组包含三个原型逆转录病毒基因，gag，pol和env，并且没有癌基因。然而，在培养的内皮细胞中，AHV通过典型的凋亡级联反应诱导显著的细胞病变效应。我们现在证明，AHV也诱导BSC-1上皮细胞和NIH-3 T3成纤维细胞的细胞增殖和锚定非依赖性生长。这通过测量（1）细胞活力、（2）DNA合成、（3）细胞DNA含量的流式细胞术分析和（4）感染细胞的克隆形成效率来显示。锚定非依赖性细胞生长证明了软琼脂中的集落形成。此外，AHV env基因被克隆到基于MuLV的逆转录病毒载体中，并且用该载体感染NIH-3 T3细胞诱导细胞增殖以及克隆形成生长。这些结果表明，缺乏致癌基因的AHV是一种多效性激活剂，能够诱导凋亡或细胞增殖，这取决于感染的细胞类型，（C）2000学术出版社。
Several years ago, a field strain retrovirus, avian hemangioma Virus (AHV), was isolated from hemangioma tumors in layer hens. Sequence analysis indicated that the AHV genome contains the three prototypic retroviral genes, gag, pol, and env, and is devoid of an oncogene. In cultured endothelial cells, however, AHV induced a significant cytopathic effect through a typical apoptotic cascade. We now demonstrate that AHV also induces cell proliferation and anchorage-independent growth of BSC-1 epithelial cells and NIH-3T3 fibroblasts. This was shown by measurements of (1) cell viability, (2) DNA synthesis, (3) flow cytometry analysis of the cell DNA content, and (4) clonogenic efficiency of the infected cells. Anchorage-independent cell growth was demonstrated by colony formation in soft agar. Moreover, the AHV env gene was cloned into a MuLV-based retroviral vector, and infection of NIH-3T3 cells with this vector induced cell proliferation as well as clonogenic growth. These results suggest that AHV, which is devoid of an oncogene, is a pleiotropic activator capable of inducing either apoptosis or cellular proliferation, depending on the infected cell type, (C) 2000 Academic Press.