An in vitro screening method for probiotics with antidepressant-like effect using the enterochromaffin cell model.
An in vitro screening method for probiotics with antidepressant-like effect using the enterochromaffin cell model.
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DOI：
10.1039/d0fo02307h
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发表时间：
2021-01
期刊：
影响因子：
6.1
通讯作者：
Peijun Tian;Huiyue Zhu;Renying Zou;Qin-ming Kong;Mengshu Xu;Jianxin Zhao;Hao Zhang;Wei Chen;Gang Wang
中科院分区：
文献类型：
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作者：
Peijun Tian;Huiyue Zhu;Renying Zou;Qin-ming Kong;Mengshu Xu;Jianxin Zhao;Hao Zhang;Wei Chen;Gang Wang
某些益生菌可以通过微生物群-肠-脑轴调节宿主的神经行为功能。然而，筛选这些益生菌主要在动物模型中进行，成本高且效率低。在本文中，假定的肠嗜铬细胞系（RIN 14 B）被用作体外预筛选模型; 30个细菌菌株进行了测试细菌刺激的色氨酸羟化酶1基因（Tph 1）的表达和5-羟色氨酸/5-羟色胺分泌。所有菌株在慢性应激诱导的抑郁小鼠中进一步验证了其神经行为效应。利用偏最小二乘法（PLS）对体外和体内数据集进行建模，发现RIN 14 B中Tph 1 mRNA水平与强迫游泳试验和蔗糖偏好试验的表现以及慢性应激小鼠血清皮质酮水平显著相关。通过对所有体内指标进行主成分分析，在30株菌株中确定了4株为最佳候选菌株，并且不出所料，其中3株能够增强RIN 14 B中的Tph 1表达，这进一步证明了基于RIN 14 B的筛选方法（特别是细菌刺激的Tph 1 mRNA的检测）对于菌株的抗抑郁样能力具有良好的预测有效性和筛选效率。总的来说，这项研究提供了一种新型的体外方法来筛选具有抗抑郁药样潜力的益生菌（或其他相关生物制品）。
Certain probiotics can regulate the host's neurobehavioral function through the microbiota-gut-brain axis. However, screening these probiotics is mainly carried out in animal models, and is costly and inefficient. Herein, a putative enterochromaffin cell line (RIN14B) was used as an in vitro pre-screening model; 30 bacterial strains were tested for bacteria-stimulated tryptophan hydroxylase 1 gene (Tph1) expression and 5-hydroxytryptophan/5-hydroxytryptamine secretion. All strains were further validated for their neurobehavioral effects in chronic stress-induced depressive mice. Using partial least squares (PLS) modeling of in vitro and in vivo datasets, we found that the level of Tph1 mRNA in RIN14B significantly correlated with the performance of a forced swim test and sucrose preference test, and serum corticosterone level in chronically stressed mice. Four strains were identified as the best candidates among 30 strains using principal component analysis on all in vivo measures, and unsurprisingly, three of them could enhance Tph1 expression in RIN14B, which further proved that the RIN14B-based screening method (especially the detection of bacteria-stimulated Tph1 mRNA) has good predictive validity and screening efficiency for the strain's antidepressant-like capacity. Collectively, this study provides a novel in vitro method for screening probiotics (or other related bioproducts) with antidepressant-like potential.