Construction of Asymmetric Cell-Sized Lipid Vesicles from Lipid-Coated Water-in-Oil Microdroplets
Construction of Asymmetric Cell-Sized Lipid Vesicles from Lipid-Coated Water-in-Oil Microdroplets
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DOI：
10.1021/jp807784j
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发表时间：
2008-11-27
影响因子：
3.3
通讯作者：
Takagi, Masahiro
中科院分区：
文献类型：
--
作者：
Hamada, Tsutomu;Miura, Yoko;Takagi, Masahiro
我们提出了一种简单，快速，和强大的方法制备不对称的细胞大小的脂质双层囊泡使用油包水（W/O）微滴通过油-水界面转移。生产细胞大小的模型膜的效率阐明有关的囊泡大小和所含的水溶性分子的重量。我们证明了生物的不对称性和形成的脂筏微区结构，使用荧光显微镜。
We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained water-soluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.