Effect of curcumin and quercetin on lysosomal enzyme activities in streptozotocin-induced diabetic rats
Effect of curcumin and quercetin on lysosomal enzyme activities in streptozotocin-induced diabetic rats
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DOI：
10.1016/j.clnu.2012.02.003
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发表时间：
2012-10-01
影响因子：
6.3
通讯作者：
Salimath, Paramahans V.
中科院分区：
文献类型：
--
作者：
Chougala, Mallikarjun B.;Bhaskar, Jamuna J.;Salimath, Paramahans V.
背景和目的：糖尿病导致生理系统中各种酶活性受损，包括溶酶体酶。饲喂姜黄素、槲皮素和氨基胍对溶酶体酶活性（即 N-乙酰基-β-D-氨基葡萄糖苷酶）的影响。对链脲佐菌素诱导的糖尿病大鼠不同组织中的β-D-葡萄糖醛酸酶、β-D-半乳糖苷酶和酸性磷酸酶进行研究。方法：将大鼠分为4个对照组和4个糖尿病组。实验组喂食补充有姜黄素（0.5%）或槲皮素（0.1%）或氨基胍（0.05%）的饮食。测定不同组织中的溶酶体酶活性。结果：与对照大鼠相比，糖尿病大鼠肝脏中 N-乙酰基-β-D-氨基葡萄糖苷酶的比活性降低，姜黄素和槲皮素治疗分别改善了 67% 和 78%。另一方面，与对照大鼠（0.45 +/- 0.02 nmol/mg 蛋白质/分钟）相比，糖尿病大鼠大脑中的 β-D-葡萄糖醛酸酶活性较高（0.90 +/- 0.04 nmol/mg 蛋白质/分钟），而姜黄素（0.75 +/- 0.05 nmol/mg 蛋白质/分钟）和槲皮素（0.74 +/- 0.11）则降低。 nmol/mg 蛋白质/分钟）治疗大鼠。与糖尿病大鼠相比，姜黄素和槲皮素喂养的糖尿病组大鼠的脾脏中β-D-半乳糖苷酶活性分别改善了68%和58%。与对照组相比，糖尿病大鼠睾丸中的酸性磷酸酶活性降低。结论：姜黄素和槲皮素喂养可调节糖尿病过程中不同组织的溶酶体酶活性，其效果与众所周知的抗糖剂氨基胍相当。 (C) 2012 Elsevier Ltd 和欧洲临床营养与代谢学会。版权所有。
Background & aims: Diabetes causes impairment of various enzyme activities in the physiological system, including lysosomal enzymes. The effect of feeding curcumin, quercetin and aminoguanidine on lysosomal enzyme activities viz., N-acetyl-beta-D-glucosaminidase. beta-D-glucuronidase, beta-D-galactosidase and acid phosphatase were studied in different tissues of streptozotocin-induced diabetic rats.Method: Rats were divided into four control groups and four diabetic groups. Experimental groups were fed with diet supplemented with curcumin (0.5%) or quercetin (0.1%) or aminoguanidine (0.05%). Lysosomal enzyme activities were determined in various tissues.Results: The specific activity of N-acetyl-beta-D-glucosaminidase in liver of diabetic rats was decreased when compared to control rats and was ameliorated with curcumin and quercetin treatment by 67% and 78%, respectively. On the other hand, beta-D-glucuronidase activity was higher in the brain of diabetic rats (0.90 +/- 0.04 nmol/mg protein/min), when compared to control rats (0.45 +/- 0.02 nmol/mg protein/min) and was decreased in curcumin (0.75 +/- 0.05 nmol/mg protein/min) and quercetin (0.74 +/- 0.11 nmol/mg protein/min) treated rats. beta-D-galactosidase activity in spleen of curcumin and quercetin fed diabetic group rats was ameliorated by 68% and 58%, respectively, in comparison to diabetic rats. Acid phosphatase activity in diabetic rats decreased in testis when compared to control.Conclusion: Curcumin and quercetin feeding modulated lysosomal enzyme activities in different tissues during diabetes and the effect was comparable to well-known anti-glycative agent - aminoguanidine. (C) 2012 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.