Cell cycle kinetics of irradiated synchronous and asynchronous tumor cells with DNA distribution analysis and BrdUrd-Hoechst 33258-technique.
Cell cycle kinetics of irradiated synchronous and asynchronous tumor cells with DNA distribution analysis and BrdUrd-Hoechst 33258-technique.
复制标题
使用 DNA 分布分析和 BrdUrd-Hoechst 33258 技术对受辐射的同步和异步肿瘤细胞进行细胞周期动力学。
DOI：
10.1002/cyto.990020206
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
M. Nüsse
中科院分区：
文献类型：
--
作者：
M. Nüsse
用流式细胞术检测同步培养的艾氏腹水癌细胞辐射诱导的S期和G2期细胞周期延迟，并分析其与照射时间的关系。用BrdUrd-Hoechst 33258技术照射异步细胞后也可以得到类似的结果。BrdUrd取代了新合成的脱氧核糖核酸中的胸苷，而胸腺嘧啶核苷特定的染料Hoechst 33258不能对其进行染色。用流式细胞仪测量了加入BrdUrd后荧光分布的时间变化。在非同步细胞中，可以计算辐射后细胞周期时相的平均持续时间及其扰动，并且数据与同步细胞中的结果一致。照射后的分裂延迟由S期的一个小进展延迟和G2期的一个阻断组成。这两种作用均依赖于细胞周期中的照射时间，受照射的晚期S细胞的S期延迟增加，受照射的G2细胞的G2期阻滞增加。这两种类型的实验数据都使用了一个简单的模型，该模型描述了照射后细胞周期阶段的细胞比例。
Radiation-induced progression delay in S-phase and G2-block, both depending on time of irradiation in the cell cycle, were measured in synchronized Ehrlich ascites tumor cells in vitro using flow cytometric analysis of DNA content in single cells. Similar results could be obtained by the BrdUrd-Hoechst 33258 technique after irradiating asynchronous cells. BrdUrd replaces thymidine in newly synthesized DNA which is not stainable by the thymidine-specific dye Hoechst 33258. The temporal development of the fluorescence distributions after addition of BrdUrd to the medium has been measured in the flow cytometer. In asynchronous cells the mean durations of the cell cycle phases and their perturbations after irradiation could be calculated, and the data agreed with the results in synchronized cells. Division delay after irradiation consists of a small progression delay in S-phase and a block in G2-phase. Both effects depend on time of irradiation during the cell cycle with delay in S-phase increasing in irradiated late S-cells and G2-block increasing in irradiated G2-cells. Data from both types of experiments were used with a simple model describing the fractions of cells in the cell cycle phases after irradiation had been applied.
高LET带电粒子诱导中国仓鼠V-79细胞细胞周期进程的初步变化。
影响因子：
3.4
作者：
Geard,CR
通讯作者：
Geard,CR