Design and simulation of a MEMS based cell separator utilizing 3D travelling-wave dielectrophoresis
Design and simulation of a MEMS based cell separator utilizing 3D travelling-wave dielectrophoresis
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利用 3D 行波介电泳设计和模拟基于 MEMS 的细胞分离器
DOI：
10.1007/s00542-015-2757-3
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
H. B. Ghavifekr
中科院分区：
文献类型：
--
作者：
Jino Fathy;A. Pourmand;H. B. Ghavifekr
在这项工作中，提出了一种新的电极结构，分离活的和非活的酵母细胞的行波介电泳（twDEP）。电极呈人字形，电极中心的锐角被曲率取代。我们采用有限元方法模拟和观察与常规介电电泳（cDEP）和twDEP相关的电场，并相应地估计所施加的电场下的细胞轨迹。此外，我们讨论了不同的物理和几何参数来设计我们的twDEP微分离器，以获得最准确的操作和更高的分离效率。首先，我们研究了适当的频率和介质电导率。然后，我们探索用于靶细胞的优化电极尺寸。从模拟中，我们发现，通过所提出的电极结构和施加振幅为2 Vp-p和频率为70 kHz的相移AC电势，活酵母细胞与非活酵母细胞区分开，并同时聚焦在通道中心的频带上。模拟结果表明，当电极宽度约为12 μm时，酵母细胞受到的twDEP力最大。最后，我们提出了可能的不同电极形式，这些电极形式可以从我们提出的电极形状导出，以操纵不同的细胞/颗粒。
In this work a novel electrode structure is proposed to separate viable and non-viable yeast cells by travelling-wave dielectrophoresis (twDEP). The electrodes are chevron-shaped which the sharp angle at the center of the electrodes is replaced by a curvature. We employed finite element method to simulate and observe the electric fields related to conventional dielectrophoresis (cDEP) and twDEP and correspondingly to estimate the cell trajectories under the applied electric field. Furthermore, we discuss different physical and geometrical parameters to design our twDEP microseparator to get the most accurate operation and higher separation efficiency. First, we study the adequate frequency and medium conductivity. Then, we explore the optimized electrode dimensions for target cells. From the simulations, we discovered that by the proposed electrode structure and applying phase shifted AC electric potential with amplitude of 2 Vp-p and frequency of 70 kHz viable yeast cells discriminate from non-viable ones and get focused on a band at the center of the channel, simultaneously. The simulation results reveal that here the maximum twDEP force is applied to yeast cells when electrode width is about 12 μm. Finally, we propose possible different electrode forms that can be derived from our proposed electrode shape to manipulate different cells/particles.
影响因子：
7.4
作者：
Grenvall C;Magnusson C;Lilja H;Laurell T
通讯作者：
Laurell T
DOI：
10.1016/j.chroma.2013.10.020
发表时间：
2013-11-29
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Li X;Xiao D;Ou XM;McCullm C;Liu YM
通讯作者：
Liu YM