Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway.
Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway.
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长链非编码RNA NEAT1通过调节miR-107/CDK6通路促进喉鳞状细胞癌
DOI：
10.1186/s13046-016-0297-z
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发表时间：
2016-01-29
期刊：
影响因子：
--
通讯作者：
Qu L
中科院分区：
文献类型：
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作者：
Wang P;Wu T;Zhou H;Jin Q;He G;Yu H;Xuan L;Wang X;Tian L;Sun Y;Liu M;Qu L
研究背景长链非编码RNA核旁斑点组装转录本1（NEAT 1）在某些人类肿瘤的发生发展中起着重要作用。然而，NEAT 1在人喉鳞状细胞癌（LSCC）中的作用仍然未知。方法采用qRT-PCR方法检测NEAT 1在喉鳞状细胞癌及癌旁组织中的表达，并分析其与喉鳞状细胞癌发生、发展的关系。在LSCC细胞中敲低NEAT 1，检测细胞增殖、凋亡和细胞周期。结果NEAT 1在喉鳞癌中的表达明显高于相应癌旁组织，且在颈淋巴结转移或临床分期较晚期的患者中表达更高。siRNA介导的NEAT 1基因敲低可显著抑制喉鳞癌细胞的增殖，诱导细胞凋亡，并使细胞周期阻滞于G1期。通过注射NEAT 1 siRNA慢病毒显著抑制了LSCC异种移植物的生长。结论NEAT 1在喉鳞状细胞癌的发生发展中起重要作用，可能成为喉鳞状细胞癌治疗的靶点。
BackgroundLong noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays key role in the progression of some human cancers. However, the role of NEAT1 in human laryngeal squamous cell cancer (LSCC) is still unknown. We therefore investigated the expression and function of NEAT1 in LSCC.MethodsNEAT1 level in LSCC and adjacent non-neoplastic tissues were detected by qRT-PCR. NEAT1 was knockdown in LSCC cells and cell proliferation, apoptosis and cell cycle were examined. The growth of xenografts with NEAT1 knockdown LSCC cells was analyzed.ResultsNEAT1 level was significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with neck nodal metastasis or advanced clinical stage had higher NEAT1 expression. Moreover, siRNA mediated NEAT1 knockdown significantly inhibited the proliferation and induced apoptosis and cell cycle arrest at G1 phase in LSCC cells. The growth of LSCC xenografts was significantly suppressed by the injection of NEAT1 siRNA lentivirus. Furthermore, NEAT1 regulated CDK6 expression in LSCC cells which was mediated by miR-107.ConclusionNEAT1 plays an oncogenic role in the tumorigenesis of LSCC and may serve as a potential target for therapeutic intervention.