Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome.
Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome.
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2 型腺病毒基因组上早期和晚期信使 RNA 序列的杂交图谱。
DOI：
10.1016/0022-2836(76)90241-2
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发表时间：
1976
影响因子：
5.6
通讯作者：
L. Philipson
中科院分区：
文献类型：
--
作者：
U. Pettersson;C. Tibbetts;L. Philipson
2 型腺病毒 DNA 的特定片段是通过限制性内切核酸酶 endR.EcoRI、endoR.HpaI 和 endR.HindIII 切割产生的，用于杂交作图实验。各个切割片段的互补链通过Tibbetts & Pettersson (1974)的方法分离。用 32 P标记的分离的切割片段链和从腺病毒感染后早期和晚期的细胞中提取的信使RNA进行液体杂交。通过羟基磷灰石色谱法测定“早期”和“晚期”信使 RNA 中各片段链的分数。人们发现早期信使RNA源自四个广泛分离的区域，两个位于1-链上，两个位于h-链上（h-和l-是指与聚（U，G）复合时在CsCl中具有重和轻浮力密度的链）。信使RNA仅在感染后晚期出现，源自多个位置，主要来自L链，其主要连续序列块在2型腺病毒基因组单位图上延伸到位置0.25和0.65之间。
Specific fragments of adenovirus type 2 DNA, generated by cleavage with restriction endonucleases endoR.EcoRI, endoR.HpaI and endoR.HindIII were used in hybridization-mapping experiments. The complementary strands of individual cleavage fragments were separated by the method of Tibbetts & Pettersson (1974). Liquid hybridizations were performed with32P-labeled separated strands of cleavage fragments and messenger RNA extracted from cells early and late after adenovirus infection. The fraction of each fragment strand which was represented in “early” and “late” messenger RNA was determined by chromatography on hydroxylapatite. Early messenger RNA was found to be derived from four widely separated regions, two on the 1- and two on the h-strand (h- and l- refer to the strand withheavyandlightbuoyant density in CsCl when complexed with poly(U, G)). Messenger RNA, present exclusively late after infection, is derived from several locations, predominantly from the l-strand with a major block of continuous sequences extending between positions 0.25 and 0.65 on the unit map of the adenovirus type 2 genome.