Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey
Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey
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DOI：
10.3390/molecules16086378
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发表时间：
2011-08-01
期刊：
影响因子：
4.6
通讯作者：
Yusof, Yasmin Anum Mohd
中科院分区：
文献类型：
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作者：
Hussein, Saba Zuhair;Yusoff, Kamaruddin Mohd;Yusof, Yasmin Anum Mohd
两种类型的单花马来西亚蜂蜜（Gelam和Nenas）进行了分析，以确定其抗氧化活性和总酚类和类黄酮含量，有和没有γ射线照射。结果表明，两种蜂蜜均具有较强的抗氧化还原能力，但Gelam蜂蜜的抗氧化活性显著高于Nenas蜂蜜（p < 0.05），且与其酚类物质含量呈良好的相关性（r = 0.9899）。有趣的是，我们还注意到，这两个辐照蜂蜜具有较高的抗氧化活性和总酚类和黄酮类化合物的含量相比，非辐照蜂蜜Folin-Ciocalteu和紫外分光光度法，分别。然而，酚类化合物的HPLC分析表明，辐照和非辐照蜂蜜之间的显着增加。通过HPLC法测定，发现Gelam蜂蜜中的酚类化合物如咖啡酸、绿原酸、鞣花酸、对香豆酸、槲皮素和橙皮素含量高于Nenas蜂蜜。总之，辐照蜂蜜导致增强的抗氧化活性和黄酮类化合物。
Two types of monofloral Malaysian honey (Gelam and Nenas) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation. Our results showed that both types of honey can scavenge free radicals and exhibit high antioxidant-reducing power; however, Gelam honey exhibited higher antioxidant activity (p < 0.05) than Nenas honey, which is in good correlation (r = 0.9899) with its phenolic contents. Interestingly, we also noted that both irradiated honeys have higher antioxidant activities and total phenolic and flavonoid contents compared to nonirradiated honeys by Folin-Ciocalteu and UV-spectrophotometry methods, respectively. However, HPLC analysis for phenolic compounds showed insignificant increase between irradiated and nonirradiated honeys. The phenolic compounds such as: caffeic acid, chlorogenic acid, ellagic acid, p-coumaric acid, quercetin and hesperetin as indicated by HPLC method were found to be higher in Gelam honey versus Nenas honey. In conclusion, irradiation of honey causes enhanced antioxidant activities and flavonoid compounds.