Human α1-Proteinase Inhibitor Binds to Extracellular Matrix In Vitro
Human α1-Proteinase Inhibitor Binds to Extracellular Matrix In Vitro
复制标题
人 α1-蛋白酶抑制剂在体外与细胞外基质结合
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
S. Simon
中科院分区：
文献类型：
--
作者：
A. Rinehart;S. Mallya;S. Simon
α1Proteinase inhibitor (α1-PI) is the major endogenous inhibitor of human leukocyte elastase (HLE). We have employed two different methods to quantitate the binding of α1-PI to extracellular matrix (ECM), composed of 51% glycoproteins and proteoglycans, 37% types I and III collagen, and 12% elastin, derived from rat heart smooth muscle cells. α1-PI is tightly bound to ECM via a saturable adsorption process; the bound protein fails to dissociate from the matrix after repeated washing. Binding of α1-PI is unaffected by the prior removal of ECM glycoproteins with trypsin. Binding to ECM is not decreased in the presence of high salt but is decreased at low pH. A 40-fold excess of unlabeled α1-PI displaces only 50% of [125I]α1-PI prebound to ECM. A 30% decrease in the levels of α1-PI bound to ECM is observed after DTT washes of ECM preincubated with α1-PI or when α1-PI is modified with iodoacetamide prior to incubation with ECM, implying that a fraction of bound α1-PI is covalently linked to ECM via disulfide ...
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DOI：
10.1073/pnas.84.16.5680
发表时间：
1987-08-01
影响因子：
11.1
作者：
INDIK, Z;YEH, H;ROSENBLOOM, J
通讯作者：
ROSENBLOOM, J
影响因子：
2.9
作者：
D. Pipoly;E. Crouch
通讯作者：
D. Pipoly;E. Crouch
基质结合纤溶酶原激活剂抑制剂 1 型抑制人单核细胞侵入间质组织。
DOI：
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发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kirchheimer,JC;Binder,BR;Remold,HG
通讯作者：
Remold,HG
DOI：
10.1172/jci114272
发表时间：
1989-10
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
R. Bar-Shavit;A. Eldor;I. Vlodavsky
通讯作者：
R. Bar-Shavit;A. Eldor;I. Vlodavsky
纤溶酶原与细胞外基质的结合。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Knudsen,BS;Silverstein,RL;Leung,LL;Harpel,PC;Nachman,RL
通讯作者：
Nachman,RL