Molecular analysis of a serotype 8 human astrovirus genome
Molecular analysis of a serotype 8 human astrovirus genome
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DOI：
10.1099/0022-1317-81-12-2891
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发表时间：
2000-12-01
影响因子：
3.8
通讯作者：
Arias, CF
中科院分区：
文献类型：
--
作者：
Méndez-Toss, M;Romero-Guido, P;Arias, CF
人类星状病毒是引起肠胃炎的重要原因。作为在墨西哥进行的分子流行病学研究的一部分，人类星状病毒分离株Yuc-8被改造成在CaCo-2细胞中生长，并对其整个基因组进行了测序。Yuc-8中存在ORF 1a中的15个氨基酸缺失，该缺失与星状病毒适应在CaCo-2以外的细胞中生长有关。Yuc-8 ORF 2与已报道的人星状病毒序列的比较序列分析显示，该分离株属于基因型（血清型）8。ORF 2中观察到两个不同的结构域：氨基末端结构域（残基1至415），在所分析的菌株中具有高于81%的同一性，和羧基末端结构域（残基416至782），具有36%至60%的同一性。两个非重叠的系统发育树，这两个域的单独分析，表明沿着结构多蛋白质施加了不同的选择压力。
Human astroviruses are an important cause of gastroenteritis. As part of a molecular epidemiological study carried out in Mexico a human astrovirus isolate, Yuc-8, was adapted to grow in CaCo-2 cells, and its entire genome was sequenced. A 15 amino acid deletion in ORF1a, which has been associated with adaptation of astroviruses to grow in cells other than CaCo-2, was present in Yuc-8. Comparative sequence analysis of the Yuc-8 ORF2 with reported human astrovirus sequences revealed that this isolate belongs to genotype (serotype) 8. Two distinct domains in ORF2 were observed:an amino-terminal domain (residues 1 to 415), with identities higher than 81% among the strains analysed, and a carboxy-terminal domain (residues 416 to 782) with identities between 36 and 60%. Two non-superimposable phylogenetic trees were generated by separate analysis of these two domains, suggesting that a differential selective pressure is exerted along the structural polyprotein.