cAMP-dependent protein kinase involves calcium tolerance through the regulation of Prz1 in Schizosaccharomyces pombe
cAMP-dependent protein kinase involves calcium tolerance through the regulation of Prz1 in Schizosaccharomyces pombe
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DOI：
10.1080/09168451.2016.1246171
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发表时间：
2017-02-01
影响因子：
1.6
通讯作者：
Kawamukai, Makoto
中科院分区：
文献类型：
--
作者：
Matsuo, Yasuhiro;Kawamukai, Makoto
CAMP依赖的蛋白激酶Pka1被认为是裂殖酵母糖合成、减数分裂和应激反应的调节因子。我们证明了Pka1与钙耐量有关。失去PKA途径的功能成分如Git3、Gpa2、Cyr1和Pka1会产生对CaCl2敏感的表型，而PKA调节亚基Cgs1的缺失会导致对CaCl2的耐受。CaCl2的加入增加了Cgs1和Pka1的胞质分布，提示CaCl2诱导了Cgs1和Pka1的解离。Prz1是一种钙稳态的转录调节因子，在葡萄糖限制的条件下，pka1 Delta株和野生型株的Prz1表达增加。因此，Prz1在野生型菌株中的高表达导致了CaCl2敏感的表型。这些发现表明，Pka1对外源CaCl2的耐受性是必不可少的，可能是因为Prz1的表达水平需要由Pka1适当地调节。
The cAMP-dependent protein kinase Pka1 is known as a regulator of glycogenesis, meiosis, and stress responses in Schizosaccharomyces pombe. We demonstrated that Pka1 is responsible for calcium tolerance. Loss of functional components of the PKA pathway such as Git3, Gpa2, Cyr1, and Pka1 yields a CaCl2-sensitive phenotype, while loss of Cgs1, a regulatory subunit of PKA, results in CaCl2 tolerance. Cytoplasmic distribution of Cgs1 and Pka1 is increased by the addition of CaCl2, suggesting that CaCl2 induces dissociation of Cgs1 and Pka1. The expression of Prz1, a transcriptional regulator in calcium homeostasis, is elevated in a pka1 Delta strain and in a wild type strain under glucose-limited conditions. Accordingly, higher expression of Prz1 in the wild type strain results in a CaCl2-sensitive phenotype. These findings suggest that Pka1 is essential for tolerance to exogenous CaCl2, probably because the expression level of Prz1 needs to be properly regulated by Pka1.