Expression and localization of epidermal growth factor, transforming growth factor-α and epidermal growth factor receptor in the canine testis.
Expression and localization of epidermal growth factor, transforming growth factor-α and epidermal growth factor receptor in the canine testis.
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DOI：
10.1262/jrd.2015-079
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Sawada T
中科院分区：
文献类型：
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作者：
Tamada H;Takemoto K;Tominaga M;Kawate N;Takahashi M;Hatoya S;Matsuyama S;Inaba T;Sawada T
本文研究了表皮生长因子（EGF）、转化生长因子-α（TGF-α）和表皮生长因子受体（EGF-R）在犬睾丸组织中的基因表达及相应蛋白的定位。用半定量逆转录聚合酶链反应（RT-PCR）方法检测了青春期（4-6个月）、青年（3-4岁）、老年（7-8岁）和衰老（11-16岁）组睾丸中mRNA的表达水平。围青春期组睾丸EGF-R mRNA水平明显高于其他组，而各组间EGF和TGF-α mRNA水平无差异。EGF、TGF-α和EGF-R免疫组化染色结果显示，睾丸生精上皮和支持细胞呈弱阳性，间质细胞呈强阳性。这些结果表明，EGF家族的生长因子可能参与睾丸成熟和功能的狗。
Gene expression of epidermal growth factor (EGF), transforming growth factor-α (TGF-α) and EGF receptor (EGF-R) and the localization of the corresponding proteins in the canine testis were studied. Levels of mRNA expressions were determined by semiquantitative reverse transcription polymerase chain reaction in the testes of the peripubertal (4–6 months), young adult (3–4 years), advanced adult (7–8 years) and senescent (11–16 years) groups. The EGF-R mRNA level in the testes of the peripubertal group was significantly higher than those in the other groups, whereas there was no difference in EGF and TGF-α mRNA levels among groups. Immunohistochemical stainings for EGF, TGF-α and EGF-R in the testis revealed that immunoreactivity in the seminiferous epithelium and Sertoli cell was weak and nonspecific for the stage of spermatogenesis, and distinct staining was found in Leydig cells. These results suggest that the EGF family of growth factors may be involved in testicular maturation and function in the dog.