Extract from Scutellaria baicalensis Georgi attenuates oxidant stress in cardiomyocytes
Extract from Scutellaria baicalensis Georgi attenuates oxidant stress in cardiomyocytes
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DOI：
10.1006/jmcc.1999.1021
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发表时间：
1999-10-01
影响因子：
5
通讯作者：
Yuan, CS
中科院分区：
文献类型：
--
作者：
Shao, ZH;Li, CQ;Yuan, CS
黄芩是一种用于治疗过敏性和炎症性疾病的中草药。黄芩根的药用作用可能部分是由于其具有抗氧化特性的组成类黄酮。由于氧化剂在细胞中扮演多种角色，我们测试了布氏链霉菌在心肌细胞缺血和再灌注模型中是否具有保护作用。用细胞内荧光探针2‘，7’-二氯二乙酸酯(DCFH-DA，对过氧化氢和羟基自由基敏感)和二氢乙锭(DHE，对超氧阴离子敏感)检测细胞内活性氧(ROS)，用碘化丙啶(PI)检测培养的胚胎心肌细胞活力。根据DCFH氧化或DHE氧化的测量，黄芩提取物(SBE)可迅速降低在短暂缺氧和暴露于线粒体III位点抑制剂抗菌素A时产生的氧化剂水平。这些减弱的氧化剂水平与改善存活率和功能有关。缺血/再灌流后细胞死亡率从未处理组的47+/-3%降至处理组的26+/-2%(P
Scutellaria baicalensis Georgi is a Chinese herbal medicine used to treat allergic and inflammatory diseases. The medicinal effects of S. baicalensis root may result, in part, from its constituent flavones reported to have antioxidant properties. Since oxidants play multiple roles in cells, we tested whether S. buicalensis could confer protection in a cardiomyocyte model of ischemia and reperfusion. The intracellular fluorescent probes 2', 7'-dichlorofluorescin diacetate (DCFH-DA, sensitive to H2O2 and hydroxyl radicals) and dihydroethidium (DHE, sensitive to superoxide) were used to assess intracellular reactive oxygen species (ROS), and propidium iodide (PI) was used to assess viability in cultured embryonic cardiomyocytes. S. baicalensis extract (SbE) quickly attenuated levels of oxidants generated during transient hypoxia and during exposure to the mitochondrial site III inhibitor antimycin A, as measured by DCFH oxidation or by DHE oxidation. These attenuated oxidant levels were associated with improved survival and function. Cell death after ischemia/ reperfusion decreased from 47+/-3% in untreated to 26+/-2% in S. baicalensis treated cells (P