Thiamine functions as a key activator for modulating plant health and broad-spectrum tolerance in cotton
Thiamine functions as a key activator for modulating plant health and broad-spectrum tolerance in cotton
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硫胺素是调节棉花植物健康和广谱耐受性的关键激活剂
DOI：
10.1111/tpj.15793
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发表时间：
2022-05-13
期刊：
影响因子：
7.2
通讯作者：
Guo, Wangzhen
中科院分区：
文献类型：
--
作者：
Li, Weixi;Mi, Xinyue;Guo, Wangzhen
全球气候变化导致非生物和生物胁迫增加，极大地威胁着世界作物安全。然而，涉及生物和非生物胁迫的广谱反应途径的研究相对较少。通过比较棉花根组织在非生物胁迫和生物胁迫条件下随时间的转录变化和共表达分析，我们发现了不同胁迫条件下共同的胁迫响应基因和胁迫代谢途径，包括昼夜节律、硫胺和半乳糖代谢、类胡萝卜素、苯丙素、类黄酮和玉米素的生物合成，以及丝裂原激活的蛋白激酶信号通路。我们发现硫胺代谢是非生物胁迫和生物胁迫的重要交汇点；硫胺合成的关键基因GhTHIC和GhTHI1在胁迫的早期阶段被高度诱导。本研究证实了硫胺素对棉花生长发育至关重要，外源补充硫胺素可以弥补硫胺素的不足。此外，我们还揭示了外源硫胺素通过增加钙信号转导和激活下游逆境响应基因来增强棉花的抗逆性。总体而言，我们的研究表明，硫胺素在植物健康和抗逆性之间的权衡中发挥了关键作用。胁迫引起的硫胺素缺乏可在体内瞬间诱导硫胺素生物合成基因的上调，而外源硫胺素可完全挽救体内硫胺素合成基因的上调，从而显著提高棉花的广谱抗逆性，促进植物生长发育。
Global climate changes cause an increase of abiotic and biotic stresses that tremendously threaten the world's crop security. However, studies on broad-spectrum response pathways involved in biotic and abiotic stresses are relatively rare. Here, by comparing the time-dependent transcriptional changes and co-expression analysis of cotton (Gossypium hirsutum) root tissues under abiotic and biotic stress conditions, we discovered the common stress-responsive genes and stress metabolism pathways under different stresses, which included the circadian rhythm, thiamine and galactose metabolism, carotenoid, phenylpropanoid, flavonoid, and zeatin biosynthesis, and the mitogen-activated protein kinase signaling pathway. We found that thiamine metabolism was an important intersection between abiotic and biotic stresses; the key thiamine synthesis genes, GhTHIC and GhTHI1, were highly induced at the early stage of stresses. We confirmed that thiamine was crucial and necessary for cotton growth and development, and its deficiency could be recovered by exogenous thiamine supplement. Furthermore, we revealed that exogenous thiamine enhanced stress tolerance in cotton via increasing calcium signal transduction and activating downstream stress-responsive genes. Overall, our studies demonstrated that thiamine played a crucial role in the tradeoff between plant health and stress resistance. The thiamine deficiency caused by stresses could transiently induce upregulation of thiamine biosynthetic genes in vivo, while it could be totally salvaged by exogenous thiamine application, which could significantly improve cotton broad-spectrum stress tolerance and enhance plant growth and development.