Tracing Mobile DNAs: From Molecular to Population Scales.
Tracing Mobile DNAs: From Molecular to Population Scales.
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DOI：
10.3389/fpls.2022.837378
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发表时间：
2022
影响因子：
5.6
通讯作者：
Cho J
中科院分区：
文献类型：
--
作者：
Fan W;Wang L;Chu J;Li H;Kim EY;Cho J
转座因子（TE、转座子）是大多数真核生物基因组中普遍存在的移动的DNA。在植物中，它们的移动性极大地促进了遗传多样性，这对物种的适应性变化和进化至关重要。转座子的这种移动的性质也已通过在非模式植物系统中产生遗传突变体而在植物科学研究中被积极地利用。另一方面，转座子动员会对宿主基因组产生不利影响，因此它们大多被表观遗传机制沉默。TEs是植物表观遗传学研究的主要沉默靶点，也是植物表观遗传学研究取得显著进展的主要特征。尽管转座子在植物生物学和生物技术中的重要性，但它们的动员和潜在机制在很大程度上没有得到解答。这主要是因为转座子的序列重复性，这使得它们的检测和分析变得困难和复杂。近年来，人们尝试开发新的实验方法来检测活性转座子及其动员行为。这些技术揭示了TE流动性在各个层面，包括分子，细胞，有机体和人口规模。本文将重点介绍移动的遗传元件研究中的新技术方法，并讨论这些技术如何影响转座子研究的进展，拓宽我们对植物基因组可塑性的理解。
Transposable elements (TEs, transposons) are mobile DNAs that are prevalent in most eukaryotic genomes. In plants, their mobility has vastly contributed to genetic diversity which is essential for adaptive changes and evolution of a species. Such mobile nature of transposon has been also actively exploited in plant science research by generating genetic mutants in non-model plant systems. On the other hand, transposon mobilization can bring about detrimental effects to host genomes and they are therefore mostly silenced by the epigenetic mechanisms. TEs have been studied as major silencing targets and acted a main feature in the remarkable growth of the plant epigenetics field. Despite the importance of transposon in plant biology and biotechnology, their mobilization and the underlying mechanisms are largely left unanswered. This is mainly because of the sequence repetitiveness of transposons, which makes their detection and analyses difficult and complicated. Recently, some attempts have been made to develop new experimental methods detecting active transposons and their mobilization behavior. These techniques reveal TE mobility in various levels, including the molecular, cellular, organismal and population scales. In this review, we will highlight the novel technical approaches in the study of mobile genetic elements and discuss how these techniques impacted on the advancement of transposon research and broadened our understanding of plant genome plasticity.
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DOI：
10.1073/pnas.93.15.7783
发表时间：
1996-07-23
影响因子：
11.1
作者：
Hirochika, H;Sugimoto, K;Kanda, M
通讯作者：
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DOI：
10.1073/pnas.88.3.936
发表时间：
1991-02-01
影响因子：
11.1
作者：
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通讯作者：
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DOI：
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发表时间：
2015-04-01
影响因子：
4.7
作者：
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通讯作者：
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影响因子：
48
作者：
Hindson, Christopher M.;Chevillet, John R.;Briggs, Hilary A.;Gallichotte, Emily N.;Ruf, Ingrid K.;Hindson, Benjamin J.;Vessella, Robert L.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh
影响因子：
64.5
作者：
HEIDMANN, O;HEIDMANN, T
通讯作者：
HEIDMANN, T