Genetic screening of the canine zinc finger protein multitype 2 (cZFPM2) gene in dogs with tetralogy of Fallot (TOF).
Genetic screening of the canine zinc finger protein multitype 2 (cZFPM2) gene in dogs with tetralogy of Fallot (TOF).
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DOI：
10.1111/j.1439-0388.2008.00776.x
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发表时间：
2009-08
期刊：
影响因子：
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通讯作者：
J-S Lee;C. Hyun
中科院分区：
文献类型：
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作者：
J-S Lee;C. Hyun
最近对人类进行的一项遗传学研究对ZFPM2基因进行了突变筛查，发现了一种杂合突变，该突变被发现与几个零星的法洛四联症(TOF)病例有关。我们假设，这也适用于患有TOF的一组狗。我们克隆了犬ZFPM2的全长cDNA，并将推导的氨基酸序列与其他物种的序列进行了比对。我们的分析表明，与小鼠或大鼠的序列相比，狗的ZFPM2在系统发育上与人类的ZFPM2更接近。进一步的基因表达分析也未能确定受影响和未受影响的狗之间的差异基因表达。我们在一组患有TOF的狗身上筛选了cZFPM2的突变，但没有发现剪接点或编码区的显著碱基变化。
A recent genetic study in humans where the ZFPM2 gene was screened for mutations revealed a heterozygous mutation that was found to contribute to several sporadic cases of tetralogy of Fallot (TOF). We hypothesized that this would also hold true for a study group of dogs with TOF. We isolated the full-length cDNA of the canine ZFPM2 and aligned the deduced amino acid sequence against sequences from other species. Our analysis indicated that the canine ZFPM2 was phylogenetically much closer to the human ZFPM2 compared with the mouse or rat sequences. Further gene expression analysis also failed to identify differential gene expression between affected and unaffected dogs. We screened the cZFPM2 for mutations in a group of dogs with TOF but found no significant base changes in either the splice sites or the coding regions.