Rhabdovirus-like endogenous viral elements in the genome of Spodoptera frugiperda insect cells are actively transcribed: Implications for adventitious virus detection.
Rhabdovirus-like endogenous viral elements in the genome of Spodoptera frugiperda insect cells are actively transcribed: Implications for adventitious virus detection.
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尖翅目frugiperda昆虫细胞基因组中类似色夫病毒样的内源性病毒元素被积极转录：对不定病毒检测的影响。
DOI：
10.1016/j.biologicals.2016.04.004
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发表时间：
2016-07
期刊：
影响因子：
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通讯作者：
Jarvis DL
中科院分区：
文献类型：
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作者：
Geisler C;Jarvis DL
狐尾夜蛾（Spodoptera frugiperda, Sf）细胞系被用于生产几种人用和兽用生物制品。最近发现，所有的Sf细胞系都持续感染了一种新型的横纹肌病毒Sf-rhabdovirus。作为寻找其他外源病毒的一部分，我们搜索了Sf细胞基因组和转录组，寻找与Sf横纹肌病毒相关的序列。令我们惊讶的是，我们发现了完整的sf -横纹肌病毒N和p样orf，以及部分sf -横纹肌病毒G和l样orf。转录物与基因组序列匹配，表明转录物来源于基因组序列。这些似乎是内源性病毒元件（EVEs），其结果是部分病毒遗传物质整合到宿主细胞基因组中。从理论上讲，Sf-rhabdovirus-like eve不可能产生感染性病毒颗粒，因为1)它们分布在4个基因组位点上，2)G和L ORF不完整，3)M ORF缺失。我们在Sf细胞中转录的病毒样序列的发现强调了基于mps在用于制造生物制剂的细胞底物中寻找外源病毒时应该同时考虑基因组和转录序列，以促进转录EVE的鉴定，并避免复制能力强的外源病毒的假阳性检测。
Spodoptera frugiperda (Sf) cell lines are used to produce several biologicals for human and veterinary use. Recently, it was discovered that all tested Sf cell lines are persistently infected with Sf-rhabdovirus, a novel rhabdovirus. As part of an effort to search for other adventitious viruses, we searched the Sf cell genome and transcriptome for sequences related to Sf-rhabdovirus. To our surprise, we found intact Sf-rhabdovirus N- and P-like ORFs, and partial Sf-rhabdovirus G- and L-like ORFs. The transcribed and genomic sequences matched, indicating the transcripts were derived from the genomic sequences. These appear to be endogenous viral elements (EVEs), which result from the integration of partial viral genetic material into the host cell genome. It is theoretically impossible for the Sf-rhabdovirus-like EVEs to produce infectious virus particles as 1) they are disseminated across 4 genomic loci, 2) the G and L ORFs are incomplete, and 3) the M ORF is missing. Our finding of transcribed virus-like sequences in Sf cells underscores that MPS-based searches for adventitious viruses in cell substrates used to manufacture biologics should take into account both genomic and transcribed sequences to facilitate the identification of transcribed EVE's, and to avoid false positive detection of replication-competent adventitious viruses.