Wood profiling by non-targeted high-resolution mass spectrometry: Part 1, Metabolite profiling in Cedrela wood for the determination of the geographical origin
Wood profiling by non-targeted high-resolution mass spectrometry: Part 1, Metabolite profiling in Cedrela wood for the determination of the geographical origin
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DOI：
10.1016/j.chroma.2021.461993
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发表时间：
2021-02-18
影响因子：
4.1
通讯作者：
Fischer, Markus
中科院分区：
文献类型：
--
作者：
Creydt, Marina;Ludwig, Lea;Fischer, Markus
确定木材的地理原产地具有很高的相关性，原因有几个：一方面，它有助于防止非法采伐和木材贸易，另一方面，它对木制考古文物以及各种生物学问题特别有意义。为此，首先对不同的提取方法进行了比较，并采用高效液相色谱-电喷雾电离四极杆飞行时间质谱仪(UHPLC-ESI-QTOF-MS)对极性和非极性代谢物进行了分析。氯仿、甲醇和水的两相萃取被证明是特别成功的。随后，对来自南美的雪松(Cedrela Odorata)样品进行了测量，以区分地理来源。利用多变量数据分析，可以提取大量与来源相关的差异。对标记物质的鉴定表明，几条代谢途径受到地理因素的影响，其中一些可能表明有害生物的侵染。？2021爱思唯尔B.V.版权所有。
The determination of the geographical origin of wood can be highly relevant for several reasons: On the one hand, it can help to prevent illegal logging and timber trade, on the other hand, it is of special interest for archaeological artefacts made of wood, as well as for a variety of biological questions. For this reason, different extraction methods were first tested for the analysis of polar and non-polar metabolites using liquid chromatography coupled electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS). A two-phase extraction with chloroform, methanol and water proved to be particularly successful. Subsequently, cedrela (Cedrela odorata) samples from South America were measured to distinguish geographic origin. Using multivariate data analysis, numerous origin-dependent differences could be extracted. The identification of the marker substances indicated that several metabolic pathways were affected by the geographical influences, some of them probably indicating pest infections. ? 2021 Elsevier B.V. All rights reserved.