Identification and evaluation of cyp1a transcript expression in fish as molecular biomarker for petroleum contamination in tropical fresh water ecosystems
Identification and evaluation of cyp1a transcript expression in fish as molecular biomarker for petroleum contamination in tropical fresh water ecosystems
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DOI：
10.1016/j.aquatox.2011.02.004
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发表时间：
2011-05-01
影响因子：
4.5
通讯作者：
Scholz, Stefan
中科院分区：
文献类型：
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作者：
dos Anjos, Nislanha Ana;Schulze, Tobias;Scholz, Stefan
为了监测乌鲁库地区（巴西）石油勘探和运输产生的潜在污染，有必要为亚马逊本地鱼类建立合适的生物标志物。因此。比较了不同潜在敏感基因（ahr2(1)、cyp1a、hmox1、hsp70、malt、mt. nfe212、gstp1和nqo1）在水溶油（WSF）中表达情况。该分析首先在一个已建立的实验室模型——斑马鱼胚胎中进行。cyp1a基因被证明是油污染最敏感和最强大的标记，因此，我们选择了cyp1a基因来研究油源污染物对亚马逊慈鲷的影响。>= 0.0061% （v/v）的wsf诱导cyp1a转录物表达。在乌鲁库油田不同湖泊采集的鱼类肝脏样本中，没有观察到cyp1a转录本的表达升高。数据表明cyp1a作为石油暴露指标的敏感性很高：应考虑进一步的研究，以测试其在已知污染地点的有效性，并通过例如中生态实验来评估影响因素。(C) 2011 Elsevier B.V.版权所有
In order to monitor potential contamination deriving from exploration and transport of oil in the Urucu region (Brazil), there is a need to establish suitable biomarkers for native Amazonian fish. Therefore. the transcript expression of various potentially sensitive genes (ahr2(1), cyp1a, hmox1, hsp70, malt, mt. nfe212,gstp1 and nqo1)in fish exposed to water soluble fractions of oil (WSF) was compared. The analysis was first performed in an established laboratory model, the zebrafish embryo. The cyp1a gene proved to be the most sensitive and robust marker for oil contamination and, hence, was selected to study the effect of oil-derived contaminants in the Amazonian cichlid Astronotus ocellatus. Induction of cyp1a transcript expression was observed for >= 0.0061% (v/v) WSFs. In liver samples of fish, collected from different lakes in the Urucu oil mining area, no elevated expression of cyp1a transcripts was observed. The data demonstrate the high sensitivity of cyp1a as indicator of oil exposure: further studies should be considered to test its usefulness at known contaminated sites and to evaluate influential factors by, e.g. mesocosm experiments. (C) 2011 Elsevier B.V. All rights reserved.