An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells.
An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells.
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哺乳动物细胞基因组激活和抑制的 CRISPR 技术简介。
DOI：
10.1101/pdb.top086835
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发表时间：
2016
影响因子：
--
通讯作者：
Qi,LeiS
中科院分区：
文献类型：
--
作者：
Du,Dan;Qi,LeiS
CRISPR 干扰/激活 (CRISPRi/a) 技术为哺乳动物基因组中基因表达的靶向抑制或激活提供了一种简单而有效的方法。它具有高度灵活性和可编程性，使用 RNA 引导的核酸酶缺陷型 Cas9 (dCas9) 蛋白与转录调节因子融合，针对特定基因来实现其调节。多项研究表明，该方法是实现单个或多个基因高效且特异性转录抑制或激活的有效方法。持续的转录调节可以通过 CRISPR 成分的稳定表达来实现，从而实现细胞命运的定向重编程。在这里，我们介绍 CRISPRi/基因组抑制或激活技术的基础知识。
CRISPR interference/activation (CRISPRi/a) technology provides a simple and efficient approach for targeted repression or activation of gene expression in the mammalian genome. It is highly flexible and programmable, using an RNA-guided nuclease-deficient Cas9 (dCas9) protein fused with transcriptional regulators for targeting specific genes to effect their regulation. Multiple studies have shown how this method is an effective way to achieve efficient and specific transcriptional repression or activation of single or multiple genes. Sustained transcriptional modulation can be obtained by stable expression of CRISPR components, which enables directed reprogramming of cell fate. Here, we introduce the basics of CRISPRi/a technology for genome repression or activation.