Distribution of immunoreactivity for P2X3, P2X5, and P2X6-purinoceptors in mouse retina
Distribution of immunoreactivity for P2X3, P2X5, and P2X6-purinoceptors in mouse retina
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DOI：
10.1007/s10735-007-9107-4
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发表时间：
2007-06
影响因子：
3.2
通讯作者：
Y. Shigematsu;Y. Shimoda;M. Kaneda
中科院分区：
文献类型：
--
作者：
Y. Shigematsu;Y. Shimoda;M. Kaneda
先前的研究表明，P2 X嘌呤受体介导的信号通路调节ACh在视网膜中的释放。我们先前报道了P2 X1-，P2 X2-，P2 X4-和P2 X7-嘌呤受体在小鼠视网膜中的免疫反应性的存在，并推测P2 X2和P2 X7-嘌呤受体可能调节胆碱能无长突细胞的活性。在本研究中，我们使用免疫组织化学技术来检查是否P2 X3-，P2 X5，P2 X6-嘌呤受体也是重要的胆碱能无长突细胞在小鼠视网膜的调制。在小鼠视网膜中观察到P2 X3-、P2 X5-和P2 X6-嘌呤受体的免疫反应性。在胆碱能无长突细胞的树突中观察到P2 X3-嘌呤受体的免疫反应性。胆碱能无长突细胞的索马胞体中存在P2 X5-嘌呤受体的免疫反应性。P2 X6-嘌呤受体免疫反应性不与胆碱能无长突细胞共定位。我们的结论是，在三个P2 X-嘌呤受体的检查，P2 X3-嘌呤受体似乎影响小鼠视网膜中的胆碱能无长突细胞的功能。
Previous findings have shown that P2X-purinoceptor-mediated signaling pathways regulate the release of ACh in the retina. We previously reported the existence of immunoreactivity for P2X1-, P2X2-, P2X4-, and P2X7-purinoceptors in mouse retina and speculated that P2X2 and P2X7-purinoceptors may modulate the activity of cholinergic amacrine cells. In the present study, we used an immunohistochemical technique to examine whether P2X3-, P2X5, and P2X6-purinoceptors are also important for the modulation of cholinergic amacrine cells in mouse retina. Immunoreactivity for P2X3-, P2X5-, and P2X6-purinoceptors was observed in mouse retina. Immunoreactivity for P2X3- purinoceptors was observed in the dendrites of cholinergic amacrine cells. Immunoreactivity for P2X5-purinoceptors existed in the soma of cholinergic amacrine cells. P2X6-purinoceptor immunoreactivity was not colocalized with the cholinergic amacrine cells. We concluded that, among the three P2X-purinoceptors that were examined, P2X3-purinoceptors seem to affect the function of cholinergic amacrine cells in the mouse retina.