Rapid Temperature-Programmed Separation and Retention Prediction on a Novel Packed-Capillary Column in Gas Chromatography
Rapid Temperature-Programmed Separation and Retention Prediction on a Novel Packed-Capillary Column in Gas Chromatography
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DOI：
10.2116/analsci.26.687
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发表时间：
2010-06
影响因子：
1.6
通讯作者：
M. Inoue;Yoshihiro Saito;I. Ueta;Tomoya Miura;H. Ohkita;K. Fujimura;K. Jinno
中科院分区：
文献类型：
--
作者：
M. Inoue;Yoshihiro Saito;I. Ueta;Tomoya Miura;H. Ohkita;K. Fujimura;K. Jinno
开发了新型气相色谱填充毛细管柱，内径为 1.0 mm 的薄壁不锈钢毛细管；在对这些色谱柱与程序升温的兼容性进行基本评估后，进行了快速程序升温分离。通过数值积分方法，成功估算了程序升温洗脱过程中多种测试分析物的保留情况。为了确认填充毛细管柱是否适合相对较快的升温速度（高达 40°C/min），将理论预测的保留数据与实际测量的数据进行了比较。结果表明，新开发的填充毛细管柱与传统使用的颗粒填充柱相比具有良好的分离性能。此外，对于目前在典型分析实验室中进行的几乎所有分离，与快速程序升温操作的兼容性是相当令人满意的。
Novel packed-capillary columns for gas chromatography were developed with a thin-wall stainless-steel capillary of 1.0 mm i.d.; and rapid temperature-programmed separations have been carried out after a basic evaluation concerning the compatibility of these columns to the temperature program. With a numerical integration method, the retention of several test analytes during temperature-programmed elution was successfully estimated. In order to confirm the suitability of the packed-capillary columns to relatively fast temperature programming up to 40°C/min, theoretically predicted retention data were compared with that actually measured. The results suggested a good separation performance of the newly developed packed-capillary columns as a particle-packed column conventionally used. Also, the compatibility to a rapid temperature-programmed operation was quite satisfactory for almost all of the separations currently done in typical analytical laboratories.