Neuroprotective effect of hypothermia at defined intraischemic time courses in cortical cultures
Neuroprotective effect of hypothermia at defined intraischemic time courses in cortical cultures
复制标题
皮质培养物中特定缺血时间过程中低温的神经保护作用
DOI：
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发表时间：
2001
影响因子：
4.2
通讯作者：
T. Mashimo
中科院分区：
文献类型：
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作者：
Sriranganathan Varathan;S. Shibuta;Tomoaki Shimizu;T. Mashimo
许多实验和临床研究表明，低温对脑缺血损伤具有脑保护作用。由于许多关于低温神经保护的报道相互矛盾，我们开展了这项细胞研究，以确定在缺血损伤期间不同时间(6-24小时)进行最大神经保护的最佳温度或温度范围。培养的Wistar大鼠皮质神经元在一定的时间和温度(37℃常温、32℃亚低温、27℃中低温、22℃深低温和17℃深低温)中暴露于缺氧。通过在光学显微镜下评估存活神经元来评估神经元的存活率。常温组培养神经元存活率(6小时，80.3%±2.7%；12小时，56.1%±2.1%；18小时，34.2%±1%；24小时，18.1%±2.2%)明显低于低温组(P<；0.001)。深低温组小鼠存活率显著低于其他低温组(17℃时：12小时、85.9%±2.5%、18小时、74.7%±3.7%、24小时、58.7%±2.7%)。轻度、中度和深低温组在缺氧18小时后的存活率几乎相同，但深低温组在缺氧24小时时存活率显著高于对照组(84.1%±1.6%；P<；0.001)。总而言之，低温对低氧有显著的神经保护作用，但与亚低温、中度低温和深低温相比，深低温对神经细胞死亡的衰减较小。与轻度和中度低温相比，深低温在长时间缺氧(>；18小时)期间为神经元提供了最大的保护。J.神经学2001年，研究结果65：583-590。©2001 Wiley-Liss公司
Many experimental and clinical studies have shown that hypothermia confers cerebroprotective benefits against ischemic insults. Because of the many conflicting reports on hypothermic neuroprotection, we undertook this cellular study to identify the optimal temperature or a range of temperatures for maximal neuroprotection at different times (6–24 hr) during ischemic insults. Cultured Wistar rat cortical neurons were exposed to oxygen deprivation at defined times and temperatures (37°C normothermia, 32°C mild hypothermia, 27°C moderate hypothermia, 22°C deep hypothermia, and 17°C profound hypothermia). The survival rate of neurons was evaluated by assessing viable neurons on photomicrographs. The normothermic group demonstrated a significantly lower survival rate of cultured neurons (6 hr, 80.3% ± 2.7%; 12 hr, 56.1% ± 2.1%; 18 hr, 34.2% ± 1%; 24 hr, 18.1% ± 2.2%) compared to hypothermic groups (P < 0.001). The survival rate for the profound hypothermic group was significantly reduced (P < 0.01) compared to other hypothermic groups (at 17°C: 12 hr, 85.9% ± 2.5%, 18 hr, 74.7% ± 3.7%, 24 hr, 58.7% ± 2.7%). Almost equal survival rates were observed among mild, moderate, and deep hypothermic groups following <18 hr exposure to hypoxia, but the deep hypothermic group showed a significantly higher survival rate (84.1% ± 1.6%; P < 0.001) when subjected to hypoxia for 24 hr. In conclusion, hypothermia offers marked neuroprotection against hypoxia, but attenuation of neuronal cell death was less with profound hypothermia compared to mild, moderate, and deep hypothermia. Deep hypothermia affords maximal protection of neurons compared to mild and moderate hypothermia during long‐lasting hypoxia (>18 hr). J. Neurosci. Res. 65:583–590, 2001. © 2001 Wiley‐Liss, Inc.
缺血内低温对可逆局灶性缺血模型脑损伤的影响。
影响因子：
4.8
作者：
Goto,Y;Kassell,NF;Hiramatsu,K;Soleau,SW;Lee,KS
通讯作者：
Lee,KS
缺血性脑损伤的温度调节——最新进展的综合。
影响因子：
--
作者：
Ginsberg,MD;Globus,MY;Dietrich,WD;Busto,R
通讯作者：
Busto,R
影响因子：
8.3
作者：
BUSTO, R;GLOBUS, MY;GINSBERG, MD
通讯作者：
GINSBERG, MD