Nitric oxide production by bone cells is fluid shear stress rate dependent
Nitric oxide production by bone cells is fluid shear stress rate dependent
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DOI：
10.1016/j.bbrc.2004.01.138
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发表时间：
2004-03-19
影响因子：
3.1
通讯作者：
Klein-Nulend, J
中科院分区：
文献类型：
--
作者：
Bacabac, RG;Smit, TH;Klein-Nulend, J
由于机械负荷引起的间质液通过腔隙-小管网络的流动而产生的剪切应力可能是骨细胞适应性反应的信号。此外，机械载荷的速率（由频率和幅度确定）决定骨形成的量。骨细胞对流体剪切应力的反应是否是速率依赖性的尚不清楚。在这里，我们调查是否骨细胞激活流体剪切应力是速率依赖性的。MC 3 T3-E1成骨细胞经受不同频率和振幅的流体剪切应力15分钟，导致峰值流体剪切应力率范围为0至39.6 Pa-Hz。一氧化氮的产生，骨细胞活化的参数，被发现是线性依赖于流体剪切应力速率;斜率是最陡的5分钟（0.11 Pa-Hz（-1）），并在15分钟下降到0.03 Pa-Hz（-1）。我们得出的结论，流体剪切应力速率是一个重要的参数或骨细胞活化。(C)2004年爱思唯尔公司All rights reserved.
Shear stress due to mechanical loading-induced flow of interstitial fluid through the lacuno-canalicular network is a likely signal for bone cell adaptive responses. Moreover, the rate (determined by frequency and magnitude) of mechanical loading determines the amount of bone formation. Whether the bone cells' response to fluid shear stress is rate dependent is unknown. Here we investigated whether bone cell activation by fluid shear stress is rate dependent. MC3T3-E1 osteoblastic cells were subjected for 15 min to fluid shear stress of varying frequencies and amplitudes, resulting in peak fluid shear stress rates ranging from 0 to 39.6 Pa-Hz. Nitric oxide production, a parameter for bone cell activation, was found to be linearly dependent on the fluid shear stress rate; the slope was steepest at 5 min (0.11 Pa-Hz(-1)) and decreased to 0.03 Pa-Hz(-1) at 15 min. We conclude that the fluid shear stress rate is an important parameter or bone cell activation. (C) 2004 Elsevier Inc. All rights reserved.