Highly selective and sensitive detection of heparin based on competition-modulated assembly and disassembly of fluorescent gold nanoclusters
Highly selective and sensitive detection of heparin based on competition-modulated assembly and disassembly of fluorescent gold nanoclusters
复制标题
基于荧光金纳米簇匕首竞争调制组装和拆卸的肝素高选择性和灵敏检测
DOI：
10.1039/c6nj03155b
复制
发表时间：
2017-01-21
影响因子：
3.3
通讯作者：
Wu, Fangying
中科院分区：
文献类型：
--
作者：
Li, Sha;Huang, Pengcheng;Wu, Fangying
We report a facile yet effective strategy for the fluorescence assay of heparin based on assembly and disassembly of the glutathione-protected gold nanoclusters ( GSH-Au NCs) modulated via the competitive interaction of cetyltrimethyl ammonium bromide ( CTAB) with heparin and GSH. Firstly, CTAB can increase the fluorescence of Au NCs by forming a nanocomposite via electrostatic and hydrophobic self-assembly. By adding heparin to the assay, CTAB is removed from the nanocomposites because of its higher affinity for heparin, quenching the fluorescence signal. Such a fluorescence feature induced via assembly and disassembly of the NCs enables our assay to possess high selectivity and sensitivity. The linear response toward heparin was obtained over the range 0.1-1.6 mu g mL(-1) with a low detection limit of 0.075 mu g mL(-1). Furthermore, satisfactory sensing perfomance for heparin in human serum makes this method hold great promise in heparin-related biomedical applications.
