Autoantibodies from a patient with scleroderma CREST recognized kinetochores of the higher plant Haemanthus.
Autoantibodies from a patient with scleroderma CREST recognized kinetochores of the higher plant Haemanthus.
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来自硬皮病患者的自身抗体 CREST 识别出高等植物 Haemanthus 的动粒。
DOI：
10.1073/pnas.87.9.3599
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发表时间：
1990
影响因子：
11.1
通讯作者：
Brinkley,BR
中科院分区：
文献类型：
--
作者：
Mole-Bajer,J;Bajer,AS;Zinkowski,RP;Balczon,RD;Brinkley,BR
来自硬皮病CREST（钙质沉着症、雷诺现象、食管运动障碍、指硬化症、毛细血管扩张症）患者的人自身抗体用于免疫染色单子叶植物Haemanthus katherinae巴克种子胚乳中染色体上的动粒。有丝分裂染色体和prekinetochores的间期细胞的着丝粒特异性染色，使用传统的间接免疫荧光程序，以及不褪色的免疫金-银增强技术，并通过荧光和视频显微镜分析。在间期，前动粒根据细胞周期的阶段是单或双结构，但在中期至晚期的前期变成四倍体（每个染色单体上有两个不同的染色点）。在前中期染色体的有利准备，多个亚基可以解决每个姐妹动粒内，这表明一个复合组织。Western blot分析表明，在HeLa和Haemanthus细胞提取物的着丝粒肽的共同表位。虽然在这两个物种中个别多肽的分子量不同，但共同表位的存在表明在整个进化过程中着丝粒/动粒组分的显著保守性。
Human autoantibodies from a patient with scleroderma CREST (calcinosis, Raynaud phenomenon, esophageal dismotility, sclerodactyly, telangiectasia) were used to immunostain kinetochores on chromosomes in endosperm of the seed of the monocot Haemanthus katherinae Bak. Kinetochores of mitotic chromosomes and prekinetochores of interphase cells were specifically stained using conventional indirect immunofluorescence procedures as well as a nonfading immunogold-silver-enhanced technique and analyzed by fluorescence and video microscopy. In interphase, prekinetochores were either single or double structures depending on the stage of the cell cycle but became quadruple (two distinct stained dots on each chromatid) in mid-to-late prophase. In favorable preparations of prometaphase chromosomes, multiple subunits could be resolved within each sister kinetochore suggesting a compound organization. Western blot analysis demonstrated common epitopes in centromeric peptides of HeLa and Haemanthus cell extracts. Although the molecular mass of individual polypeptides differed in the two species, the presence of shared epitopes indicates striking conservation of centromere/kinetochore components throughout evolution.
有丝分裂：分子和机制
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
J. Hyams;B. Brinkley
通讯作者：
B. Brinkley