Interleukin-33 facilitates neutrophil recruitment and bacterial clearance in S-aureus-caused peritonitis
Interleukin-33 facilitates neutrophil recruitment and bacterial clearance in S-aureus-caused peritonitis
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DOI：
10.1016/j.molimm.2016.03.004
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发表时间：
2016-04-01
影响因子：
3.6
通讯作者：
Yin, Hui
中科院分区：
文献类型：
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作者：
Lan, Fang;Yuan, Baohong;Yin, Hui
白细胞介素（IL）-33（IL-33）是新近发现的IL-1家族细胞因子，在Th 2相关免疫极化中起重要作用。最近越来越多的证据表明，IL-33也是抗菌感染的重要介质。在这项研究中，我们使用急性金黄色葡萄球菌腹膜感染模型研究了IL-33对抗菌反应的影响。我们的结果表明，1 L-33的管理诱导快速细菌清除和显着减少S。金黄色葡萄球菌感染相关死亡率。IL-33处理的小鼠显示出比未处理的小鼠增加的中性粒细胞流入感染病灶和腹膜中更高浓度的趋化因子CXCL 2。IL-33预激的有益作用与S.金黄色葡萄球菌诱导的中性粒细胞表面CXCR 2表达减少。此外，IL-33调节中性粒细胞导致S.金黄色葡萄球菌，这反过来又促进调理的S.金黄色。最后，IL-33刺激的中性粒细胞上调了活性氧的产生和随后对S.金黄色。总之，这些发现表明，IL-33通过调节嗜中性粒细胞介导的杀菌功能的多个步骤，在宿主抗微生物防御反应中提供了深远的影响。(C)2016爱思唯尔有限公司版权所有
Interleukin (IL)-33, a newly recognized member of IL-1 family of cytokines, plays an important role in polarizing Th2-associated immunity. Recently growing evidence indicates that IL-33 also represents a crucial mediator of antimicrobial infection. In this study, we investigated the effect of IL-33 on antibacterial response using an acute Staphylococcus aureus peritoneal infection model. Our results showed that 1L-33 administration induced a rapid bacterial clearance and markedly reduced the S. aureus infection related mortality. IL-33-treated mice displayed increased neutrophil influx into the focus of infection and higher concentrations of chemokine CXCL2 in the peritoneum than untreated mice. The beneficial effect of IL-33 priming was related to reversal of the S. aureus-induced reduction of CXCR2 expression on the surface of neutrophils. Furthermore, conditioning of neutrophils by IL-33 led to the enhancement of complement receptor 3 expression induced by S. aureus, which in turn facilitates the phagocytosis of opsonized S. aureus. Finally, neutrophils primed by IL-33 upregulated the production of reactive oxygen species and the subsequent killing activity for S. aureus. All together, these findings suggest that IL-33, through regulating multiple steps of neutrophil-mediated bactericidal function, provides a profound effect in host antimicrobial defense response. (C) 2016 Elsevier Ltd. All rights reserved.