A novel theranostic system of AS1411 aptamer-functionalized albumin nanoparticles loaded on iron oxide and gold nanoparticles for doxorubicin delivery
A novel theranostic system of AS1411 aptamer-functionalized albumin nanoparticles loaded on iron oxide and gold nanoparticles for doxorubicin delivery
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DOI：
10.1016/j.ijpharm.2019.04.025
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发表时间：
2019-06-10
影响因子：
5.8
通讯作者：
Bardania, Hassan
中科院分区：
文献类型：
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作者：
Baneshi, Marzieh;Dadfarnia, Shayessteh;Bardania, Hassan
最近，DNA适体作为可能的靶向配体引起了人们的广泛关注，因为选择性靶向癌细胞是癌症诊断和治疗的关键步骤。在此，报道了负载在氧化铁和金纳米颗粒上的 AS1411 适体功能化白蛋白纳米颗粒的开发，用于众所周知的抗癌药物阿霉素 (Dox) 的靶向递送。氧化铁纳米粒子（IONPs）和金纳米粒子（GNPs）分别通过超声辅助和控制种子生长合成方法制备。采用去溶剂交联法合成了纳米载体，并通过动态光散射、zeta电位测量、热重分析、透射电子显微镜以及振动样品磁力计进行了表征。发现合成的纳米颗粒呈球形，平均直径为 120 nm，zeta 电位约为 - 50.3 mV。通过MTT法评估所设计的运载工具对MCF7和SKBR3人癌细胞的体外抗肿瘤作用。实验结果表明，它能显着抑制癌细胞的增殖。此外，具有白蛋白涂层的GNP和IONP没有表现出任何毒性。与非靶向纳米颗粒相比，AS1411 适体功能化纳米颗粒改善了 MCF7 乳腺癌细胞的细胞摄取和效率，因为所述适体对 MCF7 细胞表面过表达的核仁素具有高亲和力。
Recently DNA aptamers have attracted remarkable attention as possible targeting ligands since selective targeting of cancer cells is a critical step in cancer diagnosis and therapy. Here, the development of AS1411 aptamer-functionalized albumin nanoparticles loaded on iron oxide and gold nanoparticles is reported for target delivery of the well-known anticancer drug of doxorubicin (Dox). Iron oxide nanoparticles (IONPs) and gold nanoparticles (GNPs) were prepared by ultrasound-assisted and controlled seeded growth synthetic methods, respectively. The nanocarrier was synthesized by a desolvation cross-linking method and characterized by dynamic light scattering, zeta potential measurement, thermogravimetric analysis, transmission electron microscopy, as well as vibrating sample magnetometer. The synthesized nanoparticles were found to be spherical with an average diameter of 120 nm and zeta potential of about - 50.3 mV. The in-vitro anti-tumor effect of the designed delivery vehicle on MCF7 and SKBR3 human cancer cells was evaluated by MTT assay. The experimental results revealed that it could significantly inhibit the proliferation of cancerous cells. Moreover, GNPs and IONPs with the coating of albumin did not show any toxicity. AS1411 aptamer-functionalized nanoparticles improved cellular uptake and efficiency to MCF7 breast cancer cells as compared to non-targeting nanoparticles because of the high affinity of mentioned aptamer toward the overexpressed nucleolin on MCF7 cell surface.