Enhancement of antigen-specific immunoglobulin G production in mice by co-administration of L-cystine and L-theanine
Enhancement of antigen-specific immunoglobulin G production in mice by co-administration of L-cystine and L-theanine
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DOI：
10.1292/jvms.69.1263
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发表时间：
2007-12-01
影响因子：
1.2
通讯作者：
Akiyama, Yukio
中科院分区：
文献类型：
--
作者：
Kurihara, Shigekazu;Shibahara, Susumu;Akiyama, Yukio
同时补充胱氨酸和谷氨酸会增加谷胱甘肽 (GSH) 的合成，与体外人巨噬细胞中单独补充任一氨基酸相比，谷胱甘肽 (GSH) 对免疫细胞功能具有显着影响。由于饮食中的谷氨酸在肠道运输过程中被代谢，口服L-茶氨酸（γ-谷氨酰乙基酰胺）主要在肝脏中代谢为谷氨酸，可以作为体内谷氨酸供体。本研究旨在调查口服 L-胱氨酸和/或 L-茶氨酸对 GSH 水平和免疫反应的影响。免疫前联合给予L-胱氨酸（200 mg/kg）和L-茶氨酸（80 mg/kg）11天，与对照小鼠的水平相比，免疫后6小时肝脏中总GSH水平显着增加。为了检查施用 L-胱氨酸和/或 L-茶氨酸对 T 辅助细胞 (Th) 1/Th2 细胞反应平衡的影响，研究了 Th1 细胞因子干扰素 (IFN)-7 和 Th2 细胞因子白细胞介素 (IL)-10 的血清比率。免疫后24小时，与对照小鼠和单次给药小鼠的比率相比，联合给药显着增加了IL-10/IFN-γ比率。此外，初次免疫前联合给药显着提高了血清抗原特异性 IgG 水平。总而言之，这些发现表明，L-胱氨酸和L-茶氨酸的共同施用部分地通过增加GSH水平和Th2介导的反应来增强抗基因特异性IgG的产生。
Supplementation with both cystine and glutamic acid increases the synthesis of glutathione (GSH), which has a marked effect on immune cell function, as compared with supplementation with either amino acid alone in human macrophages in vitro. As dietary glutamic acid is metabolized during intestinal transport, oral administration of L-theanine (gamma-glutamylethylamide), which is metabolized to glutamic acid mainly in the liver, may act as a glutamic acid donor in vivo. The present study was performed to investigate the effects of oral administration of L-eystine and/or L-theanine on GSH levels and immune responses. Co-administration of L-eystine (200 mg/kg) and L-theanine (80 mg/kg) for 11 days before immunization significantly increased the levels of total GSH in the liver 6 hr after immunization as compared with the levels in control mice. To examine the effects of administration of L-cystine and/or L-theanine on the balance of T helper (Th) 1/Th2 cell responses, the serum ratios of the Th1 cytokine, interferon (IFN)-7, and the Th2 cytokine, interleukin (IL)-10, were investigated. At 24 hr after immunization, co-administration significantly increased the IL-10/IFN-gamma ratio compared with the ratios of the control and single-administration mice. Furthermore, co-administration before primary immunization significantly enhanced serum antigen- specific IgG levels. Taken together, these findings suggest that co-administration Of L-CYstine and L-theanine enhances anti gen-speci fic IgG production partly through augmentation of GSH levels and Th2-niediated responses.