Molecular markers of clonality and identity in Epstein-Barr virus-associated B-cell lymphoproliferative disease.
Molecular markers of clonality and identity in Epstein-Barr virus-associated B-cell lymphoproliferative disease.
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Epstein-Barr 病毒相关 B 细胞淋巴增殖性疾病的克隆性和身份的分子标记。
DOI：
10.1002/jmv.20151
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Nichols,CMark
中科院分区：
文献类型：
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作者：
Walling,DennisM;Andritsos,LeslieA;Etienne,Wiguins;Payne,DeborahA;Aronson,JudithF;Flaitz,CatherineM;Nichols,CMark
EB病毒（EBV）相关B细胞淋巴增生性疾病可能是多克隆、寡克隆或单克隆疾病。肿瘤克隆性的程度反映了疾病的发病机制，并可能对疾病的诊断，预后和治疗有影响。在本研究中，分析了从免疫功能低下宿主中获得的EBV相关B细胞淋巴组织增生性疾病标本的细胞和病毒学克隆性和病毒学同一性的分子标志物。使用基于LMP-1基因序列变异的新EBV基因分型检测方法，评估每个肿瘤标本的免疫球蛋白基因JH区重排、EBV基因组末端结构和存在的EBV基因型。JH重排和EBV末端测定的结果在其肿瘤标本克隆性评估中通常是一致的，并且两种测定都有助于建立不同肿瘤标本之间的克隆同一性。EBV基因分型检测对肿瘤克隆性的评估没有显著贡献，但在从同一个体获得的不同肿瘤标本之间建立了明确的病毒学同一性。在一个个体中，这三种检测方法共同表征了多灶性单克隆肿瘤，该肿瘤可能是在连续感染两种不同的EBV基因型后通过克隆选择产生的。总之，JH重排和EBV末端检测各自提供了关于肿瘤克隆性的不同但互补的信息，而EBV基因分型检测被证明对于建立肿瘤之间的病毒学身份最有用。这三种检测方法的联合使用可能为EBV相关B细胞淋巴增生性疾病的发病机制提供新的见解。J. Med. Virol. 74：94-101，2004.© 2004 Wiley利斯公司
Epstein–Barr virus (EBV)‐associated B‐cell lymphoproliferative disease may be polyclonal, oligoclonal, or monoclonal. The degree of tumor clonality reflects the disease pathogenesis and may have implications for disease diagnosis, prognosis, and treatment. In this study, specimens of EBV‐associated B‐cell lymphoproliferative disease obtained from immunocompromised hosts were analyzed for molecular markers of cellular and virologic clonality and virologic identity. Each tumor specimen was assessed for immunoglobulin gene JH region rearrangement, the structure of the EBV genome termini, and the EBV genotype(s) present using a new EBV genotyping assay based uponLMP‐1gene sequence variation. The results of the JH rearrangement and EBV termini assays were generally concordant in their assessment of tumor specimen clonality, and both assays contributed to establishing clonal identity between different tumor specimens. The EBV genotyping assay did not significantly contribute to the assessment of tumor clonality but did established clear virologic identity between different tumor specimens obtained from the same individual. In one individual, these three assays together characterized a multi‐focal, monoclonal tumor that may have arisen through clonal selection after sequential infections with two different EBV genotypes. In summary, the JH rearrangement and EBV termini assays each provided different but complementary information on tumor clonality, while the EBV genotyping assay proved most useful for establishing virologic identity among tumors. Utilization of these three assays together may provide new insight into the pathogenesis of EBV‐associated B‐cell lymphoproliferative disease. J. Med. Virol. 74:94–101, 2004. © 2004 Wiley‐Liss, Inc.