NmeSI restriction-modification system identified by representational difference analysis of a hypervirulent Neisseria meningitidis strain
NmeSI restriction-modification system identified by representational difference analysis of a hypervirulent Neisseria meningitidis strain
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DOI：
10.1128/iai.69.3.1816-1820.2001
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发表时间：
2001-03-01
影响因子：
3.1
通讯作者：
van der Ende, A
中科院分区：
文献类型：
--
作者：
Bart, A;Pannekoek, Y;van der Ende, A
脑膜炎奈瑟氏菌是一种革兰氏阴性细菌，可能导致脑膜炎、败血症或两者兼而有之。在过去的20年中，脑膜炎球菌病在各国的发病率增加主要是由于基因型相关的谱系III脑膜炎球菌。用代表性差异分析法鉴定了Ⅲ系菌株和非Ⅲ系菌株之间的染色体DNA差异。因此，一个1.8 kb的基因座，是特定的谱系III脑膜炎球菌被确定。该基因座含有编码NmeSI限制修饰系统的三个开放阅读框。克隆了甲基转移酶基因并在大肠杆菌中表达。发现AGTACT位点被酶修饰。总之，谱系III菌株与地方性菌株的不同之处在于存在特定的限制修饰系统。这种限制修饰系统可能有助于通过影响水平基因转移和转录的谱系III菌株的克隆和高毒力的字符。
Neisseria meningitidis is a gram-negative bacterium that may cause meningitis, sepsis, or both. The increase in the incidence of meningococcal disease in various countries in the past 2 decades is mainly due the genotypically related lineage III meningococci. The chromosomal DNA differences between lineage III strains and non-lineage III strains were identified using representational difference analysis. Thus, a 1.8-kb locus that is specific for lineage III meningococci was identified. The locus contains three open reading frames encoding the NmeSI restriction-modification system. The methyltransferase gene was cloned and expressed in Escherichia coli. Site AGTACT was found to be modified by the enzyme. In conclusion, lineage III strains differ from endemic strains by the presence of a specific restriction-modification system. This restriction-modification system may contribute to the clonal and hypervirulent character of lineage III strains by influencing horizontal gene transfer and transcription.