The Structure of Gene Product 6 of Bacteriophage T4, the Hinge-Pin of the Baseplate
The Structure of Gene Product 6 of Bacteriophage T4, the Hinge-Pin of the Baseplate
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DOI：
10.1016/j.str.2009.04.005
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发表时间：
2009-06-10
期刊：
影响因子：
5.7
通讯作者：
Rossmann, Michael G.
中科院分区：
文献类型：
--
作者：
Aksyuk, Anastasia A.;Leiman, Petr G.;Rossmann, Michael G.
T4噬菌体的基板是一种多组分蛋白复合物，控制噬菌体与宿主的附着。它由六个楔子和一个中心枢纽组装而成。在感染期间，基板经历了巨大的构象变化，从圆顶形状变为扁平的星形结构。我们报道了基因产物（gp） 6的c端一半的晶体结构，并研究了它在基板重排过程中相对于其他蛋白质的运动。六个gp6二聚体相互交错，形成一个环，在两种构象中保持基板的完整性。一个基板楔包含gp6的n端二聚体，而邻近的楔通过gp6的c端二聚体连接在一起。二聚体相互作用在整个基板重排过程中保持不变。然而，gp6的N端和c端零件之间的铰链角变化类似于15度，占gp6环直径径向增加10埃。
The baseplate of bacteriophage T4 is a multicomponent protein complex, which controls phage attachment to the host. It assembles from six wedges and a central hub. During infection the baseplate undergoes a large conformational change from a dome-shaped to a flat, star-shaped structure. We report the crystal structure of the C-terminal half of gene product (gp) 6 and investigate its motion with respect to the other proteins during the baseplate rearrangement. Six gp6 dimers interdigitate, forming a ring that maintains the integrity of the baseplate in both conformations. One baseplate wedge contains an N-terminal dimer of gp6, whereas neighboring wedges are tied together through the C-terminal dimer of gp6. The dimeric interactions are preserved throughout the rearrangement of the baseplate. However, the hinge angle between the N- and C-terminal parts of gp6 changes by similar to 15 degrees, accounting for a 10 angstrom radial increase in the diameter of the gp6 ring.