Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development
Cloning and characterization of a calcium dependent protein kinase gene associated with cotton fiber development
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DOI：
10.1007/s00299-008-0603-0
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发表时间：
2008-12-01
影响因子：
6.2
通讯作者：
Xia, Gui-Xian
中科院分区：
文献类型：
--
作者：
Huang, Quan-Sheng;Wang, Hai-Yun;Xia, Gui-Xian
从棉花中鉴定出钙依赖性蛋白激酶基因GhCPK1。GhCPK1转录本主要在伸长纤维中积累，用GhCPK1启动子-GUS结构转化的拟南芥植株主要在发育中的毛状体、根、幼叶和花萼中表现出GUS活性。在轰击洋葱表皮细胞中，GhCPK1-GFP融合蛋白在质膜中呈亚细胞分布。体外实验表明，GhCPK1是一种功能性钙依赖性激酶，能够进行自磷酸化和已知底物组蛋白III-S的磷酸化。综上所述，这些结果表明GhCPK1可能在与纤维伸长相关的钙信号事件中发挥作用。
The gene GhCPK1 encoding a calcium dependent protein kinase was identified from cotton. Transcripts of GhCPK1 accumulated primarily in the elongating fiber, and Arabidopsis plants transformed with GhCPK1 promoter-GUS construct exhibited GUS activity mainly in the developing trichomes, roots, young leaves and sepals. In the bombarded onion epidermal cells, GhCPK1-GFP fusion proteins showed a subcellular distribution in the plasma membrane. In vitro assays indicated that GhCPK1 was a functional calcium-dependent kinase able to undergo autophosphorylation and phosphorylation of the known substrate histone III-S. Together, these results suggest that GhCPK1 may play a role in the calcium signaling events associated with fiber elongation.